Garcia-Alvarado J S, Labbé R G, Rodriguez M A
Departamento de Microbiologia, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico.
Appl Environ Microbiol. 1992 Apr;58(4):1411-4. doi: 10.1128/aem.58.4.1411-1414.1992.
Enterotoxin-positive strains of Clostridium perfringens were grown in Duncan-Strong sporulation medium in the presence of 0.4% (7.9 mM) raffinose at 37 and 43 degrees C. Enterotoxin- and heat-resistant spores were produced at similar concentrations but sooner at 43 degrees C than at 37 degrees C. There was a direct relationship between spore heat resistance and sporulation temperature (32, 37, and 43 degrees C).
产气荚膜梭菌的肠毒素阳性菌株在含有0.4%(7.9 mM)棉子糖的邓肯-斯特朗芽孢形成培养基中于37℃和43℃培养。产生的肠毒素和耐热芽孢浓度相似,但在43℃时比在37℃时产生得更快。芽孢耐热性与芽孢形成温度(32℃、37℃和43℃)之间存在直接关系。