用电免疫扩散法定量产气荚膜梭菌A型肠毒素
Quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion.
作者信息
Duncan C L, Somers E B
出版信息
Appl Microbiol. 1972 Nov;24(5):801-4. doi: 10.1128/am.24.5.801-804.1972.
Abstract
Conditions for quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion are described. As little as 0.01 mug of enterotoxin could be detected. Electroimmunodiffusion was more sensitive than either single gel diffusion or quantitation based on erythemal activity of the toxin in guinea pig skin.
摘要
本文描述了通过电免疫扩散法定量A型产气荚膜梭菌肠毒素的条件。该方法能够检测低至0.01微克的肠毒素。电免疫扩散法比单向凝胶扩散法或基于毒素在豚鼠皮肤中的红斑活性的定量法更为灵敏。
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