Ali A E, Arakawa O, Noguchi T, Miyazawa K, Shida Y, Hashimoto K
Laboratory of Marine Biochemistry, Faculty of Agriculture, University of Tokyo, Japan.
Toxicon. 1990;28(9):1083-93. doi: 10.1016/0041-0101(90)90147-y.
A ribbon worm Cephalothrix linearis (Nemertean) showed a high toxicity, of up to 22,000 MU/g proboscis and 13,600 MU/g body, in terms of tetrodotoxin. This organism secreted the toxin from the skin when wiped with gauze. The toxin was partially purified from the secretion as well as the wiped body by ultrafiltration and Bio-Gel P-2 column chromatography. Thin-layer chromatographic, electrophoretic, high performance liquid chromatographic, and gas chromatography-mass spectrometric analyses showed that the secreted toxin was composed almost exclusively of a tetrodonic acidic-like substance, whereas the remaining toxin in the wiped body consisted of the said substance and tetrodotoxin.
一种带状蠕虫——线形头吻纽虫（纽形动物门），就河豚毒素而言，显示出高毒性，吻部高达22,000鼠单位/克，身体高达13,600鼠单位/克。用纱布擦拭这种生物时，它会从皮肤分泌毒素。通过超滤和生物凝胶P - 2柱色谱法从分泌物以及擦拭后的虫体中对毒素进行了部分纯化。薄层色谱、电泳、高效液相色谱和气相色谱 - 质谱分析表明，分泌的毒素几乎完全由一种类河豚酸物质组成，而擦拭后的虫体中剩余的毒素则由该物质和河豚毒素组成。
