Miyazawa K, Higashiyama M, Ito K, Noguchi T, Arakawa O, Shida Y, Hashimoto K
Faculty of Applied Biological Science, Hiroshima University, Japan.
Toxicon. 1988;26(9):867-74. doi: 10.1016/0041-0101(88)90327-3.
Extracts of two species of ribbon worm, Lineus fuscoviridis and Tubulanus punctatus, had lethal potencies in mice from 15-503 MU/g of worm and from less than 10-540 MU/g, respectively. The toxins were partially purified by ultrafiltration and column chromatography using Bio-Gel P-2 and Bio-Rex 70 (H+ form). Thin-layer chromatographic, high performance liquid chromatographic and gas chromatography-mass spectrometric analyses revealed the presence of tetrodotoxin and anhydrotetrodotoxin plus some unidentified compounds.
两种纽虫，即褐绿纽虫（Lineus fuscoviridis）和斑点管纽虫（Tubulanus punctatus）的提取物，对小鼠的致死效力分别为每克虫体15 - 503鼠单位和低于10 - 540鼠单位。毒素通过超滤以及使用生物凝胶P - 2和生物雷克斯70（H⁺型）的柱色谱法进行了部分纯化。薄层色谱、高效液相色谱和气相色谱 - 质谱分析表明存在河豚毒素和脱水河豚毒素以及一些未鉴定的化合物。
