Department of Neurosurgery, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
PLoS One. 2012;7(5):e36016. doi: 10.1371/journal.pone.0036016. Epub 2012 May 11.
In the adult rodent brain, neural progenitor cells migrate from the subventricular zone of the lateral ventricle towards the olfactory bulb in a track known as the rostral migratory stream (RMS). To facilitate the study of neural progenitor cells and stem cell therapy in large animal models of CNS disease, we now report the location and characteristics of the normal canine and feline RMS. The RMS was found in Nissl-stained sagittal sections of adult canine and feline brains as a prominent, dense, continuous cellular track beginning at the base of the anterior horn of the lateral ventricle, curving around the head of the caudate nucleus and continuing laterally and ventrally to the olfactory peduncle before entering the olfactory tract and bulb. To determine if cells in the RMS were proliferating, the thymidine analog 5-bromo-2-deoxyuridine (BrdU) was administered and detected by immunostaining. BrdU-immunoreactive cells were present throughout this track. The RMS was also immunoreactive for markers of proliferating cells, progenitor cells and immature neurons (Ki-67 and doublecortin), but not for NeuN, a marker of mature neurons. Luxol fast blue and CNPase staining indicated that myelin is closely apposed to the RMS along much of its length and may provide guidance cues for the migrating cells. Identification and characterization of the RMS in canine and feline brain will facilitate studies of neural progenitor cell biology and migration in large animal models of neurologic disease.
在成年啮齿动物大脑中，神经祖细胞从侧脑室的室下区迁移到嗅球，沿着一条被称为嗅球迁移流（RMS）的轨迹前进。为了促进中枢神经系统疾病的大动物模型中神经祖细胞和干细胞治疗的研究，我们现在报告正常犬和猫 RMS 的位置和特征。在成年犬和猫大脑的尼氏染色矢状切片中，可以发现 RMS 是一条突出的、密集的、连续的细胞轨迹，从侧脑室前角的基部开始，绕过尾状核头部，向外侧和腹侧延伸至嗅球进入嗅球之前的嗅球。为了确定 RMS 中的细胞是否增殖，给予胸苷类似物 5-溴-2-脱氧尿苷（BrdU）并通过免疫染色检测。该轨迹中存在 BrdU 免疫反应性细胞。RMS 还对增殖细胞、祖细胞和未成熟神经元的标志物（Ki-67 和双皮质蛋白）呈免疫反应性，但对成熟神经元的标志物 NeuN 呈阴性。洛索夫快速蓝和 CNPase 染色表明，髓磷脂在其大部分长度上与 RMS 紧密相邻，可能为迁移细胞提供导向线索。犬和猫大脑中 RMS 的鉴定和特征描述将促进神经祖细胞生物学和神经疾病大动物模型中迁移的研究。
