Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, 88302-202 Itajaí, SC, Brazil ; Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, 88302-202 Itajaí, SC, Brazil.
Evid Based Complement Alternat Med. 2013;2013:265025. doi: 10.1155/2013/265025. Epub 2013 Jun 9.
This study evaluated extracts, fractions, and isolated compounds from some selected Brazilian medicinal plants against strains of promastigotes of Leishmania amazonensis and L. brasiliensis in vitro. The cell viability was determined, comparing the results with reference standards. The dichloromethane fractions of the roots, stems, and leaves of Allamanda schottii showed IC50 values between 14.0 and 2.0 μ g/mL. Plumericin was the main active compound, with IC50 of 0.3 and 0.04 μ g/mL against the two species of Leishmania analyzed. The hexane extract of Eugenia umbelliflora fruits showed IC50 of 14.3 and 5.7 μ g/mL against L. amazonensis and L. brasiliensis, respectively. The methanolic extracts of the seeds of Garcinia achachairu and guttiferone A presented IC50 values of 35.9 and 10.4 μ g/mL, against L. amazonensis, respectively. The ethanolic extracts of the stem barks of Rapanea ferruginea and the isolated compound, myrsinoic acid B, presented activity against L. brasiliensis with IC50 of 24.1 and 6.1 μ g/mL. Chloroform fraction of Solanum sisymbriifolium exhibited IC50 of 33.8 and 20.5 μ g/mL, and cilistol A was the main active principle, with IC50 of 6.6 and 3.1 μ g/mL against L. amazonensis and L. brasiliensis, respectively. It is concluded that the analyzed plants are promising as new and effective antiparasitic agents.
本研究评估了一些巴西药用植物的提取物、馏分和分离化合物对美洲钩虫和巴西钩虫的体外原虫的抑制作用。通过比较结果与参比标准，确定了细胞活力。Allamanda schottii 的根、茎和叶的二氯甲烷馏分显示出的 IC50 值在 14.0 到 2.0μ g/mL 之间。普卢米林是主要的活性化合物，对两种分析的利什曼原虫的 IC50 值分别为 0.3 和 0.04μ g/mL。Eugenia umbelliflora 果实的正己烷提取物对 L. amazonensis 和 L. brasiliensis 的 IC50 值分别为 14.3 和 5.7μ g/mL。Garcinia achachairu 的种子的甲醇提取物和 guttiferone A 呈现出的 IC50 值分别为 35.9 和 10.4μ g/mL，分别针对 L. amazonensis。Rapanea ferruginea 的茎树皮的乙醇提取物和分离的化合物 myrsinoic acid B 对 L. brasiliensis 的活性具有 IC50 值分别为 24.1 和 6.1μ g/mL。Solanum sisymbriifolium 的氯仿馏分显示出的 IC50 值分别为 33.8 和 20.5μ g/mL，cilistol A 是主要的活性成分，对 L. amazonensis 和 L. brasiliensis 的 IC50 值分别为 6.6 和 3.1μ g/mL。可以得出结论，所分析的植物具有作为新型有效抗寄生虫药物的潜力。
