逆转录聚合酶链反应/电喷雾电离质谱法（PLEX-ID/流感检测法）对流感阳性标本的初步评估
Pilot Evaluation of RT-PCR/Electrospray Ionization Mass Spectrometry (PLEX-ID/Flu assay) on Influenza-Positive Specimens.
作者信息
Cordey Samuel, Thomas Yves, Suter Patricia, Kaiser Laurent
机构信息
Swiss National Reference Centre for Emerging Viral Diseases, University of Geneva Hospitals, Geneva, Switzerland.
出版信息
Open Virol J. 2012;6:64-7. doi: 10.2174/1874357901206010064. Epub 2012 May 9.
Abstract
The PLEX-ID/Flu assay has been recently developed to enable the detection and typing of influenza viruses based on the RT-PCR/electrospray ionization mass spectrometry technology.This novel assay was evaluated for typing performance on 201 positive influenza A or B nasopharyngeal swab specimens (NPS) detected by real-time RT-PCR during the 2010-2011 season. The PLEX-ID/Flu assay detected and characterized 91.3% and 95.3% of all influenza A and B samples, respectively. All non-typeable influenza A and B specimens by the assay showed low viral loads with threshold cycle values ≥ 33. Taken together, and although our results need to be confirmed by further prospective studies, the PLEX-ID/Flu assay detected positively and gave a typing result for 93% of all NPS detected positively by real-time RT-PCR, thus suggesting a potential role for influenza virus surveillance among other techniques.
摘要
PLEX-ID/流感检测法最近得以开发，用于基于逆转录聚合酶链反应/电喷雾电离质谱技术检测流感病毒并进行分型。对2010 - 2011流感季期间通过实时逆转录聚合酶链反应检测出的201份甲型或乙型流感病毒阳性鼻咽拭子样本（NPS），评估了这种新型检测法的分型性能。PLEX-ID/流感检测法分别检测并鉴定出了91.3%的甲型流感样本和95.3%的乙型流感样本。该检测法所有无法分型的甲型和乙型样本均显示病毒载量较低，阈值循环值≥33。总体而言，尽管我们的结果需要通过进一步的前瞻性研究来证实，但PLEX-ID/流感检测法对实时逆转录聚合酶链反应检测出的所有阳性NPS中的93%检测呈阳性并给出了分型结果，因此表明在其他技术中，该检测法在流感病毒监测方面具有潜在作用。
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