Cordey Samuel, Thomas Yves, Suter Patricia, Kaiser Laurent
Swiss National Reference Centre for Emerging Viral Diseases, University of Geneva Hospitals, Geneva, Switzerland.
Open Virol J. 2012;6:64-7. doi: 10.2174/1874357901206010064. Epub 2012 May 9.
The PLEX-ID/Flu assay has been recently developed to enable the detection and typing of influenza viruses based on the RT-PCR/electrospray ionization mass spectrometry technology.This novel assay was evaluated for typing performance on 201 positive influenza A or B nasopharyngeal swab specimens (NPS) detected by real-time RT-PCR during the 2010-2011 season. The PLEX-ID/Flu assay detected and characterized 91.3% and 95.3% of all influenza A and B samples, respectively. All non-typeable influenza A and B specimens by the assay showed low viral loads with threshold cycle values ≥ 33. Taken together, and although our results need to be confirmed by further prospective studies, the PLEX-ID/Flu assay detected positively and gave a typing result for 93% of all NPS detected positively by real-time RT-PCR, thus suggesting a potential role for influenza virus surveillance among other techniques.
PLEX-ID/流感检测法最近得以开发，用于基于逆转录聚合酶链反应/电喷雾电离质谱技术检测流感病毒并进行分型。对2010 - 2011流感季期间通过实时逆转录聚合酶链反应检测出的201份甲型或乙型流感病毒阳性鼻咽拭子样本（NPS），评估了这种新型检测法的分型性能。PLEX-ID/流感检测法分别检测并鉴定出了91.3%的甲型流感样本和95.3%的乙型流感样本。该检测法所有无法分型的甲型和乙型样本均显示病毒载量较低，阈值循环值≥33。总体而言，尽管我们的结果需要通过进一步的前瞻性研究来证实，但PLEX-ID/流感检测法对实时逆转录聚合酶链反应检测出的所有阳性NPS中的93%检测呈阳性并给出了分型结果，因此表明在其他技术中，该检测法在流感病毒监测方面具有潜在作用。
