巴西土壤和植物中细菌对氟乙酸钠的脱氟作用
Defluorination of sodium fluoroacetate by bacteria from soil and plants in Brazil.
作者信息
Camboim Expedito K A, Tadra-Sfeir Michelle Z, de Souza Emanuel M, Pedrosa Fabio de O, Andrade Paulo P, McSweeney Chris S, Riet-Correa Franklin, Melo Marcia A
机构信息
Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande, Avenida Universitária, s/n, Bairro Sta. Cecília, Patos, PB, CEP: 58700-970, Brazil.
出版信息
ScientificWorldJournal. 2012;2012:149893. doi: 10.1100/2012/149893. Epub 2012 Apr 24.
The aim of this work was to isolate and identify bacteria able to degrade sodium fluoroacetate from soil and plant samples collected in areas where the fluoroacetate-containing plants Mascagnia rigida and Palicourea aenofusca are found. The samples were cultivated in mineral medium added with 20 mmol L(-1) sodium fluoroacetate. Seven isolates were identified by 16S rRNA gene sequencing as Paenibacillus sp. (ECPB01), Burkholderia sp. (ECPB02), Cupriavidus sp. (ECPB03), Staphylococcus sp. (ECPB04), Ancylobacter sp. (ECPB05), Ralstonia sp. (ECPB06), and Stenotrophomonas sp. (ECPB07). All seven isolates degraded sodium-fluoroacetate-containing in the medium, reaching defluorination rate of fluoride ion of 20 mmol L(-1). Six of them are reported for the first time as able to degrade sodium fluoroacetate (SF). In the future, some of these microorganisms can be used to establish in the rumen an engineered bacterial population able to degrade sodium fluoroacetate and protect ruminants from the poisoning by this compound.
这项工作的目的是从在发现含氟乙酸植物硬叶马斯卡尼亚草和暗色帕利古里亚草的地区采集的土壤和植物样本中分离并鉴定能够降解氟乙酸钠的细菌。将样本在添加了20 mmol L⁻¹氟乙酸钠的矿物培养基中培养。通过16S rRNA基因测序鉴定出七株菌株，分别为芽孢杆菌属（ECPB01）、伯克霍尔德菌属（ECPB02）、贪铜菌属（ECPB03）、葡萄球菌属（ECPB04）、弯曲杆菌属（ECPB05）、罗尔斯通氏菌属（ECPB06）和嗜麦芽窄食单胞菌属（ECPB07）。所有七株菌株都降解了培养基中的含氟乙酸钠，使氟离子的脱氟率达到20 mmol L⁻¹。其中六株首次被报道能够降解氟乙酸钠（SF）。未来，这些微生物中的一些可用于在瘤胃中建立能够降解氟乙酸钠并保护反刍动物免受该化合物中毒的工程细菌群体。
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