Seigneurin J M, Scherrer R, Baccard-Longere M, Genoulaz O, Feynerol C, Gout J P
Biomedicine. 1979 Jul;31(4):99-104.
ELISA and negative contrast EM have used for the detection of rotavirus in one hundred stools from children, less than two years old, hospitalized for acute gastro-enteritis during the winter 1977-78. Samples obtained from the same children some months after hospitalization were also tested. A good correlation was found between the results given by EM and ELISA, but the later technique turned out to be more sensitive (12% more positive using ELISA). A rotavirus infection could be demonstrated in 73% of the patients. In the stools of 3 children we found a second virus in association with the rotavirus, and in two cases a pathogenic bacterium. When a second serum specimen was available from children previously infected by rotavirus it was always possible to detect a significant increase in CF antibodies. Several months after hospitalization a 2nd survey indicated that the rotavirus was no longer present but calicivirus, echovirus, coxsackievirus and adenovirus could be detected in those asymptomatic children.
酶联免疫吸附测定（ELISA）和阴性对照电子显微镜检查法被用于检测1977 - 1978年冬季因急性肠胃炎住院的100名两岁以下儿童粪便中的轮状病毒。还对这些儿童在住院数月后采集的样本进行了检测。结果发现电子显微镜检查法和酶联免疫吸附测定法的结果具有良好的相关性，但后一种技术更为灵敏（酶联免疫吸附测定法的阳性率高出12%）。73%的患者被证实感染了轮状病毒。在3名儿童的粪便中，我们发现除轮状病毒外还有另一种病毒，在两例中还发现了一种致病细菌。当有曾感染轮状病毒儿童的第二份血清样本时，总能检测到补体结合（CF）抗体显著增加。住院数月后的第二次调查表明，轮状病毒已不再存在，但在那些无症状儿童中可检测到杯状病毒、艾柯病毒、柯萨奇病毒和腺病毒。
