Parra Pessoa Igor, Lopes Neto José Joaquim, Silva de Almeida Thiago, Felipe Farias Davi, Vieira Leonardo Rogério, Lima de Medeiros Jackeline, Augusti Boligon Aline, Peijnenburg Ad, Castelar Ivan, Fontenele Urano Carvalho Ana
Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil.
Department of Molecular Biology, Federal University of Paraíba, 58051-900 João Pessoa, PB, Brazil.
Molecules. 2016 Dec 21;21(12):1755. doi: 10.3390/molecules21121755.
Studies have shown the benefit of antioxidants in the prevention or treatment of human diseases and promoted a growing interest in new sources of plant antioxidants for pharmacological use. This study aimed to add value to two underexploited wild plant species ( and ) from Brazilian flora. Thus, the phenolic compounds profile of their seed ethanol extract and derived fractions were elucidated by HPLC, the antioxidant capacity was assessed by in vitro chemical tests and the cytotoxicity determined using the human carcinoma cell lines MCF-7 and Caco-2. Eleven phenolic compounds were identified in the extracts of each species. The extracts and fractions showed excellent antioxidant activity in the DPPH assay (SC, ranging from 9.15 to 248.8 µg/mL). The aqueous fraction of seeds was most effective in preventing lipid peroxidation under basal conditions (IC 60.80 µg/mL) whereas, in the presence of stress inducer, the methanolic fraction of performed best (IC 8.55 µg/mL). None of the samples showed iron chelating capacity. Ethanolic seed extracts of both species did not reveal any cytotoxicity against MCF-7 and Caco-2 cells. Both plant species showed a promising phenolic profile with potent antioxidant capacity and deserve attention to be sustainably explored.
研究表明抗氧化剂在预防或治疗人类疾病方面具有益处，并促使人们对用于药理学的植物抗氧化剂新来源越来越感兴趣。本研究旨在提升巴西植物区系中两种未得到充分利用的野生植物物种（和）的价值。因此，通过高效液相色谱法阐明了它们种子乙醇提取物及其衍生馏分的酚类化合物谱，通过体外化学测试评估了抗氧化能力，并使用人癌细胞系MCF-7和Caco-2测定了细胞毒性。在每个物种的提取物中鉴定出11种酚类化合物。提取物和馏分在DPPH测定中表现出优异的抗氧化活性（SC，范围为9.15至248.8μg/mL）。种子的水相馏分在基础条件下预防脂质过氧化方面最有效（IC60.80μg/mL），而在存在应激诱导剂的情况下，的甲醇相馏分表现最佳（IC8.55μg/mL）。所有样品均未显示出铁螯合能力。两种物种的种子乙醇提取物对MCF-7和Caco-2细胞均未显示出任何细胞毒性。这两种植物物种均显示出具有前景的酚类谱，具有强大的抗氧化能力，值得关注以便进行可持续开发。
