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[某种水果]提取物对三氧化二砷诱导的人淋巴细胞DNA氧化损伤的抗氧化作用及保护潜力。 （注：原文中“of ”后面缺少具体水果名称）

Antioxidant effects and protective potential of fruit extracts of against arsenic trioxide-induced human lymphocytes DNA oxidative damages.

作者信息

Rouamba Ablassé, Ouedraogo Vincent, Ouédraogo Maurice, Kiendrebeogo Martin

机构信息

Laboratoire de Biochimie et Chimie Appliquées (LABIOCA), UFR-SVT, Université Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso.

Laboratoire de Physiologie Animale (LAPA), UFR-SVT, Université Joseph KI-ZERBO, 09 BP 848, Ouagadougou 09, Burkina Faso.

出版信息

Avicenna J Phytomed. 2025 Jan-Feb;15(1):837-847. doi: 10.22038/AJP.2024.24842.

DOI:10.22038/AJP.2024.24842
PMID:40271503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12013971/
Abstract

OBJECTIVE

The integrity of the DNA is continously menaced by the harmful genotoxic compounds. The endogenous system responsible for preserving the DNA integrity, often fails following a massive influx of these genotoxic compounds. Reseaches on exogenous bioactive compounds from fruits and vegetables are necessary. This study was designed to evaluate the free radical scavenging activity and the DNA protection/repair potentiality of the extracts of fruit pulp to protect against the arsenic trioxide-induced DNA oxidative degradation.

MATERIALS AND METHODS

The ability of extracts to trap free radicals was assessed by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide and hydroxyle radicals quenching assay. The comet assay was performed for evaluating the DNA protection/repair property of extracts to inhibit the DNA oxidative damage induced by arsenic trioxide.

RESULTS

All extracts at a final concentration of 50 µg/mL have quenched more than 50% of DPPH, nitric oxide and hydroxyle radicals. Moreover, all extracts have showed good DNA protection/repair activity against the arsenic trioxide-induced DNA oxidative damage compared to arsenic treatment alone (p<0.001). However, methanol fractions have exhibited the best DNA protection/repair activities by reducing considerably DNA fragmentations compared to arsenic treatment (p<0.001). The genoprotective activity of the extracts was positively correlated with their free radical scavenging abilities.

CONCLUSION

The methanol fraction of fruits have exhibited interesting DNA protection /repair properties probably due to its free radicals quenching ability. Further investigations are necessary to identify the phytomolecules responsible for these biological activities.

摘要

目的

DNA的完整性不断受到有害基因毒性化合物的威胁。负责维持DNA完整性的内源性系统，在这些基因毒性化合物大量涌入后常常失效。因此，有必要对来自水果和蔬菜的外源性生物活性化合物进行研究。本研究旨在评估果肉提取物的自由基清除活性以及DNA保护/修复潜力，以防止三氧化二砷诱导的DNA氧化降解。

材料与方法

通过使用2,2-二苯基-1-苦基肼（DPPH）、一氧化氮和羟基自由基淬灭试验来评估提取物捕获自由基的能力。采用彗星试验评估提取物抑制三氧化二砷诱导的DNA氧化损伤的DNA保护/修复特性。

结果

所有最终浓度为50μg/mL的提取物均淬灭了超过50%的DPPH、一氧化氮和羟基自由基。此外，与单独砷处理相比，所有提取物均显示出对三氧化二砷诱导的DNA氧化损伤具有良好的DNA保护/修复活性（p<0.001）。然而，与砷处理相比，甲醇组分通过显著减少DNA片段化表现出最佳的DNA保护/修复活性（p<0.001）。提取物的基因保护活性与其自由基清除能力呈正相关。

结论

水果的甲醇组分表现出有趣的DNA保护/修复特性，这可能归因于其自由基淬灭能力。有必要进一步研究以确定负责这些生物活性的植物分子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/32de0aa7a231/AJP-15-837-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/624d51f3aa75/AJP-15-837-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/1ebcab8665e7/AJP-15-837-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/75db61f607b5/AJP-15-837-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/0cb79ef3209c/AJP-15-837-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/32de0aa7a231/AJP-15-837-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/624d51f3aa75/AJP-15-837-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/1ebcab8665e7/AJP-15-837-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/75db61f607b5/AJP-15-837-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/0cb79ef3209c/AJP-15-837-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45c/12013971/32de0aa7a231/AJP-15-837-g005.jpg

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