通过两个遗传掺入的非规范氨基酸指导的蛋白质无催化剂和位点特异性一锅双标记。
Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids.
机构信息
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
出版信息
Chembiochem. 2012 Jul 9;13(10):1405-8. doi: 10.1002/cbic.201200281. Epub 2012 May 24.
Abstract
The genetic incorporation of one azide-containing and one keto-containing noncanonical amino acids into a protein at one amber and one ochre mutation sites respectively followed by their orthogonal reactions with one hydroxylamine-containing and one cyclooctyne-containing dyes allows highly efficient one-pot site-specific dual labeling of the protein in a catalyst-free fashion.
摘要
将一个含叠氮基和一个含酮基的非天然氨基酸分别遗传掺入到一个琥珀突变和一个赭色突变的蛋白质中，然后分别与一个含羟胺基和一个含环辛炔基的染料进行正交反应，可在无催化剂的条件下高效地一锅法进行蛋白质的位点特异性双重标记。
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