采用 Bio-Plex® 平台分析子宫内膜异位症患者腹腔液中的细胞因子随月经周期阶段的变化。
Analysis of cytokines in the peritoneal fluid of endometriosis patients as a function of the menstrual cycle stage using the Bio-Plex® platform.
机构信息
Department of Clinical Research, University of Berne, Switzerland.
出版信息
Arch Physiol Biochem. 2012 Oct;118(4):210-8. doi: 10.3109/13813455.2012.687003. Epub 2012 May 26.
OBJECTIVE
Endometriosis is a painful disease affecting 10-15% of reproductive-age women. Concentrations of several cytokines and angiogenic factors in peritoneal fluid (PF) have been found to correlate with the severity of the disease. However, levels of some analytes vary across the menstrual cycle, and an ideal biomarker of endometriosis has not yet been identified. We have compared the PF concentrations of different cytokines in proliferative and secretory phases in women with and without the disease using the Bio-Plex platform.
METHODS
PF was aspirated during laparoscopy (N = 133) and the PF concentrations of 18 cytokines from Bio-Plex panels I and II determined with the serum protocol.
RESULTS
Increased PF concentrations of IL-6, IL-18, eotaxin, and MCP-1 were found in endometriosis with no changes with menstrual cycle. Levels of IL-12(p70), ICAM-1, and GRO-α were higher in the secretory phase, while eotaxin concentrations were lower.
CONCLUSION
Of the 18 cytokines tested, IL-6, IL-18 and MCP-1 were the best PF markers of endometriosis.
目的
子宫内膜异位症是一种影响 10-15%育龄期女性的疼痛性疾病。已发现腹腔液（PF）中几种细胞因子和血管生成因子的浓度与疾病的严重程度相关。然而，一些分析物的水平在月经周期中会发生变化，并且尚未确定子宫内膜异位症的理想生物标志物。我们使用 Bio-Plex 平台比较了有和没有该疾病的女性在增殖期和分泌期 PF 中不同细胞因子的浓度。
方法
在腹腔镜检查期间抽吸 PF（N = 133），并使用血清方案确定来自 Bio-Plex 面板 I 和 II 的 18 种细胞因子的 PF 浓度。
结果
在子宫内膜异位症中发现 PF 中 IL-6、IL-18、嗜酸性粒细胞趋化因子和 MCP-1 的浓度增加，且与月经周期无关。IL-12(p70)、ICAM-1 和 GRO-α 的水平在分泌期较高，而嗜酸性粒细胞趋化因子的浓度较低。
结论
在测试的 18 种细胞因子中，IL-6、IL-18 和 MCP-1 是 PF 中子宫内膜异位症的最佳标志物。
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