DNA 的合成、组装及其在合成生物学中的应用。
DNA synthesis, assembly and applications in synthetic biology.
机构信息
Department of Biomedical Engineering and the Institute for Genome Sciences and Policy, Duke University, Durham, NC 27708, USA.
出版信息
Curr Opin Chem Biol. 2012 Aug;16(3-4):260-7. doi: 10.1016/j.cbpa.2012.05.001. Epub 2012 May 25.
Abstract
The past couple of years saw exciting new developments in microchip-based gene synthesis technologies. Such technologies hold the potential for significantly increasing the throughput and decreasing the cost of gene synthesis. Together with more efficient enzymatic error correction and genome assembly methods, these new technologies are pushing the field of synthetic biology to a higher level.
摘要
过去几年，基于微芯片的基因合成技术取得了令人兴奋的新进展。这些技术有可能显著提高基因合成的通量并降低成本。与更有效的酶纠错和基因组组装方法相结合，这些新技术正在将合成生物学领域推向一个更高的水平。
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本文引用的文献
1
Error correction in gene synthesis technology.基因合成技术中的纠错。
Trends Biotechnol. 2012 Mar;30(3):147-54. doi: 10.1016/j.tibtech.2011.10.002. Epub 2011 Dec 28.
2
Error correction of microchip synthesized genes using Surveyor nuclease.利用 Surveyor 核酸内切酶纠正微芯片合成基因中的错误。
Nucleic Acids Res. 2012 Feb;40(3):e23. doi: 10.1093/nar/gkr887. Epub 2011 Nov 29.
3
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement.精确地在体内操纵染色体可实现全基因组密码子替换。
Science. 2011 Jul 15;333(6040):348-53. doi: 10.1126/science.1205822.
4
Automated assembly of standard biological parts.标准生物部件的自动化组装
Methods Enzymol. 2011;498:363-97. doi: 10.1016/B978-0-12-385120-8.00016-4.
5
Gene synthesis: methods and applications.基因合成：方法与应用
Methods Enzymol. 2011;498:277-309. doi: 10.1016/B978-0-12-385120-8.00012-7.
6
Parallel on-chip gene synthesis and application to optimization of protein expression.平行芯片上基因合成及其在蛋白质表达优化中的应用。
Nat Biotechnol. 2011 May;29(5):449-52. doi: 10.1038/nbt.1847. Epub 2011 Apr 24.
7
Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries.环形聚合酶延伸克隆技术用于高通量克隆复杂和组合 DNA 文库。
Nat Protoc. 2011 Feb;6(2):242-51. doi: 10.1038/nprot.2010.181. Epub 2011 Feb 3.
8
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Integr Biol (Camb). 2011 Feb;3(2):109-18. doi: 10.1039/c0ib00070a. Epub 2011 Jan 19.
9
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