State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China 102206.
Diagn Microbiol Infect Dis. 2012 Aug;73(4):301-7. doi: 10.1016/j.diagmicrobio.2012.04.004. Epub 2012 May 25.
The rapid and reliable identification of pathogens is crucial for confirming infections concomitant with community-acquired pneumonia (CAP), guiding antimicrobial therapy, and epidemiologic surveillance. In this study, 2 matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems coupled to the Biotyper or SARAMIS database were used to identify strains isolated from the throat swab samples of 70 CAP patients. The analysis of 16S rRNA gene sequencing was used as the reference method. A total of 212 suspicious colonies representing 12 genera and 30 species were identified. Of these, 99.1% (total 210/212 and 202/212 in Biotyper and 193/212 in SARAMIS) were successfully identified with 93.4% (total 198 /212 and 190/212 in Biotyper and 149/212 in SARAMIS) identified at the species level. The integrity and comprehensiveness of the databases are the main reason for the significant differences in the identification of isolates between the Biotyper and SARAMIS systems. As a rapid, economical, and high-throughput method, MALDI-TOF MS is an effective alternative identification method that can aid in the diagnosis and surveillance of CAP.
快速、可靠地鉴定病原体对于确定社区获得性肺炎（CAP）合并感染、指导抗菌治疗和流行病学监测至关重要。本研究使用两种基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统与 Biotyper 或 SARAMIS 数据库联用，鉴定了 70 例 CAP 患者咽拭子样本中分离的菌株。16S rRNA 基因测序分析被用作参考方法。共鉴定出代表 12 属和 30 种的 212 个可疑菌落。其中，99.1%（Biotyper 中总共有 210/212 个和 202/212 个，SARAMIS 中总共有 193/212 个）成功鉴定，93.4%（Biotyper 中总共有 198/212 个和 190/212 个，SARAMIS 中总共有 149/212 个）在种水平上鉴定。Biotyper 和 SARAMIS 系统在鉴定分离株方面存在显著差异，主要原因是数据库的完整性和全面性不同。MALDI-TOF MS 作为一种快速、经济、高通量的方法，是一种有效的替代鉴定方法，可辅助 CAP 的诊断和监测。
