Department of Chemical, Materials & Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA.
J Mater Sci Mater Med. 2012 Sep;23(9):2177-86. doi: 10.1007/s10856-012-4682-7. Epub 2012 May 26.
Parathyroid hormone (PTH) is a well-known therapeutic agent for osteoporosis treatment, however, the inconvenience of daily administration and side effect from systematic administration severely limits its application in clinic. PTH has been incorporated into a biomimetic calcium phosphate (CaP) coating via a co-precipitation method in a modified simulated body fluid. The aim of the current study is to evaluate the osseointegration response of PTH incorporated CaP coating on titanium implants. Implants with different doses of PTH were inserted into tibiae of mice and evaluated by X-ray, micro-CT, histology and back-scattered scanning electron microscopy. Improved osseointegration of the implants loaded with PTH was observed compared to CaP coating only after 28 days of implantation in mouse tibiae. Micro-CT analysis showed better bone integration around the implant incorporated with PTH. Bone area and bone contact evaluations have demonstrated that peri-implant bone regeneration is highly dependent on the dosage of PTH incorporated. The higher the PTH content, the more bone formed surrounding the implant. Therefore, our results suggest that biomimetic CaP coating could be a useful a carrier for PTH local delivery, which results in improved bone-to-implant integration.
甲状旁腺激素 (PTH) 是治疗骨质疏松症的一种众所周知的治疗药物，然而，其日常给药的不便和系统给药的副作用严重限制了其在临床中的应用。PTH 通过共沉淀方法被掺入到通过改良的模拟体液合成的仿生磷酸钙 (CaP) 涂层中。本研究的目的是评估 PTH 掺入的 CaP 涂层对钛植入物的骨整合反应。将不同剂量 PTH 的植入物插入到小鼠的胫骨中，并通过 X 射线、微 CT、组织学和背散射扫描电子显微镜进行评估。与仅用 CaP 涂层相比，在植入小鼠胫骨 28 天后，负载 PTH 的植入物的骨整合得到了改善。微 CT 分析显示，在 PTH 植入物周围有更好的骨整合。骨面积和骨接触评估表明，植入物周围的骨再生高度依赖于掺入的 PTH 剂量。PTH 含量越高，植入物周围形成的骨越多。因此，我们的结果表明，仿生 CaP 涂层可以作为 PTH 局部递送的有用载体，从而提高骨与植入物的整合。
