Plant Pathology Department, University of Nebraska-Lincoln, 204 Morrison Hall, Lincoln, NE 68583-0900, USA.
Can J Microbiol. 2012 Jun;58(6):788-801. doi: 10.1139/w2012-052. Epub 2012 May 29.
Curtobacterium flaccumfaciens pv. flaccumfaciens is a Gram-positive bacterium and has reemerged as an incitant of bacterial wilt in common (dry, edible) beans in western Nebraska, eastern Colorado, and southeastern Wyoming. Curtobacterium flaccumfaciens pv. flaccumfaciens is diverse phenotypically and genotypically and is represented by several different pathogen color variants. The population structure of 67 strains collected between 1957 and 2009, including some isolated from alternate hosts, was determined with 3 molecular typing techniques: amplified fragment length polymorphism (AFLP), repetitive extragenic palindromic polymerase chain reaction (rep-PCR), and pulsed-field gel electrophoresis (PFGE). All 3 typing techniques showed a great degree of population heterogeneity, but they were not congruent in cluster analysis of the C. flaccumfaciens pv. flaccumfaciens populations. Cluster analysis of a composite data set (AFLP, PFGE, and rep-PCR) using averages from all experiments yielded 2 distinct groups: cluster A included strains with colonies of yellow, orange, and pink pigments, and cluster B had strains of only yellow pigment. Strains producing purple extracellular pigment were assigned to both clusters. Thus, C. flaccumfaciens pv. flaccumfaciens is diverse phenotypically and genotypically.
短稳杆菌致病变种是一种革兰氏阳性细菌，已重新成为内布拉斯加州西部、科罗拉多州东部和怀俄明州东南部常见（干制、可食用）豆类细菌性萎蔫病的病原体。短稳杆菌致病变种在表型和基因型上具有多样性，并由几种不同的病原菌颜色变体代表。1957 年至 2009 年间收集的 67 株菌的种群结构，包括一些从替代宿主中分离的菌，用 3 种分子分型技术进行了确定：扩增片段长度多态性（AFLP）、重复外回文聚合酶链反应（rep-PCR）和脉冲场凝胶电泳（PFGE）。所有 3 种分型技术均显示出高度的种群异质性，但在短稳杆菌致病变种种群的聚类分析中并不一致。使用所有实验的平均值对复合数据集（AFLP、PFGE 和 rep-PCR）进行聚类分析，产生了 2 个不同的组：组 A 包括具有黄色、橙色和粉红色色素菌落的菌株，组 B 仅具有黄色色素的菌株。产生紫色细胞外色素的菌株被分配到这 2 个组中。因此，短稳杆菌致病变种在表型和基因型上均具有多样性。
