Galimov Sh N, Gromenko D S, Galimova É F, Gromenko Iu Iu, Iskhakov I R
Urologiia. 2012 Jan-Feb(1):47-51.
The aim of our investigation was detection of molecular mechanisms of an antioxidant action of L-carnitine (a transmembrane carrier of long-chain fatty acids) in idiopathic pathospermia. We treated 60 males from infertile couples with karniton (1 g/day). L-carnitine impact on ejaculate antioxidant status was assessed by registration of chemiluminescence and total antioxidant activity by colorimetric method according to changes in the color of ABTS chromogen. High dose-dependent antioxidant activity of the drug was found in testing on model systems in vitro. In vivo trials showed that patients given L-carnitine demonstrated a significant increase of the number of mobile spermatozoa of A category, positive changes in parameters of luminal-dependent and Fe2+ induced chemiluminescence of ejaculate in elevated antioxidant capacity of spermoplasma. After karniton treatment of the husbands 23% of their wives got pregnant. The treatment efficacy may be due to normalization of antioxidant status of ejaculate.
我们研究的目的是检测左旋肉碱（长链脂肪酸的跨膜载体）在特发性弱精子症中抗氧化作用的分子机制。我们用卡尼丁（1克/天）治疗了60对不育夫妇中的男性。根据ABTS显色剂颜色变化，通过比色法记录化学发光和总抗氧化活性，评估左旋肉碱对精液抗氧化状态的影响。在体外模型系统测试中发现该药物具有高剂量依赖性抗氧化活性。体内试验表明，给予左旋肉碱的患者精液中A类活动精子数量显著增加，精液中管腔依赖性和Fe2+诱导的化学发光参数出现积极变化，精子质的抗氧化能力增强。丈夫接受卡尼丁治疗后，23%的妻子怀孕。治疗效果可能归因于精液抗氧化状态的正常化。
