Centre for DNA Nanotechnology at the Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus C, Denmark.
J Am Chem Soc. 2012 Jun 20;134(24):9844-7. doi: 10.1021/ja3017939. Epub 2012 Jun 7.
The DNA origami approach enables the construction of complex objects from DNA strands. A fundamental understanding of the kinetics and thermodynamics of DNA origami assembly is extremely important for building large DNA structures with multifunctionality. Here both experimental and theoretical studies of DNA origami melting were carried out in order to reveal the reversible association/disassociation process. Furthermore, by careful control of the temperature cycling via in situ thermally controlled atomic force microscopy, the self-assembly process of a rectangular DNA origami tile was directly visualized, unveiling key mechanisms underlying their structural and thermodynamic features.
DNA 折纸方法使人们能够用 DNA 链构建复杂的物体。对于构建具有多功能性的大型 DNA 结构，深入了解 DNA 折纸组装的动力学和热力学非常重要。在这里，通过实验和理论研究了 DNA 折纸的熔化过程，以揭示其可逆的结合/解离过程。此外，通过原位热控原子力显微镜对温度循环的精细控制，直接观察到矩形 DNA 折纸瓦片的自组装过程，揭示了它们的结构和热力学特性的关键机制。
