Levitz S M, DiBenedetto D J, Diamond R D
Evans Memorial Department of Clinical Research, Boston University Medical Center, MA 02118.
Antonie Van Leeuwenhoek. 1990 Aug;58(2):107-14. doi: 10.1007/BF00422726.
Both components of the polyamine oxidase (PAO)-polyamine system are known to be present in phagocytes and have thus been postulated to contribute to the antimicrobial activity of these cells. Therefore, the effects of the PAO-polyamine system on three medically important opportunistic fungi were examined. Yeasts of Cryptococcus neoformans, but not Candida albicans blastoconidia or Aspergillus fumigatus conidia, were efficiently killed by the system. Two putative end products of the system, hydrogen peroxide and acrolein, both killed C. neoformans at concentrations attainable with the whole system. However, catalase failed to inhibit activity of the whole system, making hydrogen peroxide an unlikely mediator of killing. Although C. albicans blastoconidia and A. fumigatus conidia were not killed by the PAO-polyamine system, germ tube formation by the former, and hyphal growth by the latter, were markedly inhibited. These data establish that the PAO-polyamine system possesses antifungal activity.
已知多胺氧化酶（PAO）-多胺系统的两个组成部分都存在于吞噬细胞中，因此据推测它们有助于这些细胞的抗菌活性。因此，研究了PAO-多胺系统对三种医学上重要的机会性真菌的影响。新型隐球菌酵母能被该系统有效杀死，而白色念珠菌芽生孢子或烟曲霉分生孢子则不能。该系统的两种假定终产物过氧化氢和丙烯醛，在整个系统可达到的浓度下都能杀死新型隐球菌。然而，过氧化氢酶未能抑制整个系统的活性，这使得过氧化氢不太可能是杀伤的介质。虽然白色念珠菌芽生孢子和烟曲霉分生孢子未被PAO-多胺系统杀死，但前者的芽管形成和后者的菌丝生长均受到明显抑制。这些数据表明PAO-多胺系统具有抗真菌活性。
