Rzepczyk C M, Saul A J, Ferrante A
Infect Immun. 1984 Jan;43(1):238-44. doi: 10.1128/iai.43.1.238-244.1984.
The polyamines spermine and spermidine, in the presence of polyamine oxidase, were shown to be cytotoxic in vitro to various isolates of Plasmodium falciparum. Neither polyamines nor polyamine oxidase alone was cytotoxic. This cytotoxicity was manifested by the degeneration of the parasites into crisis forms and by the inhibition of methionine incorporation by the parasites. Only 2 to 2.5 h of exposure to the reaction mixture (polyamine oxidase, 100 micrograms/ml; spermine, 1 mM) resulted in parasite death. It was shown that ammonia, hydrogen peroxide, and associated reactive oxygen intermediates produced during the oxidation of polyamines were not the cause of the parasite death observed in this system. This suggested that aldehydes or further breakdown products of these, e.g., acrolein (or both), need to be considered as the effector substances of the polyamine oxidase-mediated killing of P. falciparum.
在多胺氧化酶存在的情况下，多胺精胺和亚精胺在体外对恶性疟原虫的各种分离株显示出细胞毒性。单独的多胺或多胺氧化酶都没有细胞毒性。这种细胞毒性表现为寄生虫退化为危机形式以及寄生虫对蛋氨酸掺入的抑制。仅暴露于反应混合物（多胺氧化酶，100微克/毫升；精胺，1毫摩尔）2至2.5小时就会导致寄生虫死亡。结果表明，多胺氧化过程中产生的氨、过氧化氢和相关的活性氧中间体不是该系统中观察到的寄生虫死亡的原因。这表明醛或其进一步的分解产物，例如丙烯醛（或两者），需要被视为多胺氧化酶介导的恶性疟原虫杀伤的效应物质。
