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靶向递药的新型工具：适体。

Escort aptamers: new tools for the targeted delivery of therapeutics into cells.

机构信息

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences.

出版信息

Acta Naturae. 2011 Oct;3(4):12-29.

PMID:22649701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3347615/
Abstract

Escort aptamers are DNA or RNA sequences with high affinity to certain cell-surface proteins, which can be used for targeted delivery of various agents into cells of a definite type. The peculiarities of the selection of escort aptamers are discussed in this review. The methods used in selection of escort aptamers via the SELEX technique are considered, including selection against isolated cell-surface proteins, cell fragments, living eukaryotic cells, and bacteria. Particular attention is given to the design and chemical modification of escort aptamers. The different fields of application of escort aptamers are described, including the targeted delivery of siRNAs, nanoparticles, toxins, and photoagents, as well as the identification of specific cell markers and the detection or isolation of cells of a definite type. The potential for the application of escort aptamers in the development of new therapeutic agents and diagnostic systems is also discussed.

摘要

引导适体是与特定细胞表面蛋白具有高亲和力的 DNA 或 RNA 序列，可用于将各种试剂靶向递送至特定类型的细胞。本综述讨论了引导适体选择的特点。考虑了通过 SELEX 技术选择引导适体所使用的方法，包括针对分离的细胞表面蛋白、细胞片段、活真核细胞和细菌的选择。特别关注引导适体的设计和化学修饰。描述了引导适体的不同应用领域，包括 siRNA、纳米颗粒、毒素和光剂的靶向递送，以及特定细胞标记物的鉴定和特定类型细胞的检测或分离。还讨论了引导适体在开发新的治疗剂和诊断系统中的应用潜力。

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本文引用的文献

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2
A multivalent DNA aptamer specific for the B-cell receptor on human lymphoma and leukemia.一种针对人淋巴瘤和白血病 B 细胞受体的多价 DNA 适体。
Nucleic Acids Res. 2011 Mar;39(6):2458-69. doi: 10.1093/nar/gkq996. Epub 2010 Oct 28.
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Advances in aptamers.适体的进展。
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Therapeutic aptamers: developmental potential as anticancer drugs.治疗性适配体：作为抗癌药物的发展潜力。
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Aptamers: novel molecules as diagnostic markers in bacterial and viral infections?适体：新型分子作为细菌和病毒感染的诊断标志物？
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Selection and characterization of DNA aptamers with binding selectivity to Campylobacter jejuni using whole-cell SELEX.采用全细胞 SELEX 技术筛选并鉴定对空肠弯曲菌具有结合选择性的 DNA 适体。
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