Aseervatham G Smilin Bell, Sivasudha T, Sasikumar J M, Christabel P Hephzibah, Jeyadevi R, Ananth D Arul
Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India.
J Physiol Biochem. 2014 Mar;70(1):1-14. doi: 10.1007/s13105-013-0274-3. Epub 2013 Jul 20.
Pouteria campechiana (Kunth) Baehni. is used as a remedy for coronary trouble, liver disorders, epilepsy, skin disease, and ulcer. Therefore, the present study aims to investigate the antioxidant and hepatoprotective effect of polyphenolic-rich P. campechiana fruit extract against acetaminophen-intoxicated rats. Total phenolic and flavonoid contents of egg fruit were estimated followed by the determination of antioxidant activities. Treatment with P. campechiana fruit extract effectively scavenged the free radicals in a concentration-dependent manner within the range of the given concentrations in all antioxidant models. The presence of polyphenolic compounds were confirmed by high-performance thin-layer chromatography (HPTLC). The animals were treated with acetaminophen (250 mg/kg body weight; p.o.) thrice at the interval of every 5 days after the administration of P. campechiana aqueous extract and silymarin (50 mg/kg). Acetaminophen treatment was found to trigger an oxidative stress in liver, leading to an increase of serum marker enzymes. However, treatment with P. campechiana fruit extract significantly reduced the elevated liver marker enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) and increased the antioxidant enzymes (viz., superoxide dismutase and catalase) and glutathione indicating the effect of the extract in restoring the normal functional ability of hepatocytes. These results strongly suggest that P. campechiana fruit extract has strong antioxidant and significant hepatoprotective effect against acetaminophen-induced hepatotoxicity.
坎佩切蛋黄果（Pouteria campechiana (Kunth) Baehni.）被用作治疗冠心病、肝脏疾病、癫痫、皮肤病和溃疡的药物。因此，本研究旨在探讨富含多酚的坎佩切蛋黄果果实提取物对乙酰氨基酚中毒大鼠的抗氧化和肝保护作用。先测定了蛋黄果的总酚和黄酮含量，然后测定其抗氧化活性。在所有抗氧化模型中，坎佩切蛋黄果果实提取物处理均能在给定浓度范围内以浓度依赖的方式有效清除自由基。通过高效薄层色谱法（HPTLC）确认了多酚类化合物的存在。在给予坎佩切蛋黄果水提取物和水飞蓟宾（50 mg/kg）后，每隔5天给动物口服三次乙酰氨基酚（250 mg/kg体重）。发现乙酰氨基酚处理会引发肝脏氧化应激，导致血清标志物酶升高。然而，坎佩切蛋黄果果实提取物处理显著降低了升高的肝脏标志物酶（天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶），并增加了抗氧化酶（即超氧化物歧化酶和过氧化氢酶）以及谷胱甘肽，表明该提取物对恢复肝细胞正常功能能力有作用。这些结果有力地表明，坎佩切蛋黄果果实提取物具有强大的抗氧化作用，对乙酰氨基酚诱导的肝毒性具有显著的肝保护作用。
