不同类别海藻硫酸多糖抗登革热病毒的活性。

Antiviral activity against dengue virus of diverse classes of algal sulfated polysaccharides.

机构信息

Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina.

出版信息

Int J Biol Macromol. 2012 Nov;51(4):412-6. doi: 10.1016/j.ijbiomac.2012.05.028. Epub 2012 May 28.

DOI:10.1016/j.ijbiomac.2012.05.028

PMID:22652218

Abstract

Diverse classes of sulfated polysaccharides obtained from the red seaweeds (Rhodophyta) Grateloupia indica, Scinaia hatei and Gracilaria corticata, the brown seaweeds (Phaeophyta) Stoechospermum marginatum and Cystoseira indica and the green seaweed (Chlorophyta) Caulerpa racemosa were assayed for antiviral activity against the four serotypes of dengue virus (DENV). DENV-2 was the most susceptible serotype to all polysulfates, with inhibitory concentration 50% values in the range 0.12-20 μg/mL. The antiviral potency of the sulfated polysaccharides depended on the sulfate content, the position of sulfate group, the sugar composition, and the molar mass. Independently of the sugar composition, the antiviral effect was mainly exerted during DENV-2 adsorption and internalization.

摘要

从红海藻（红藻门）Grateloupia indica、Scinaia hatei 和 Gracilaria corticata、褐海藻（褐藻门）Stoechospermum marginatum 和 Cystoseira indica 以及绿海藻（绿藻门）Caulerpa racemosa 中获得的不同种类的硫酸化多糖对四种血清型登革热病毒（DENV）进行了抗病毒活性检测。DENV-2 是对所有多硫酸盐最敏感的血清型，抑制浓度 50% 值在 0.12-20 μg/mL 范围内。硫酸化多糖的抗病毒效力取决于硫酸根含量、硫酸根位置、糖组成和摩尔质量。与糖组成无关，抗病毒作用主要在 DENV-2 吸附和内化过程中发挥。