Department of Oral and Maxillofacial Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
Int J Oral Maxillofac Surg. 2012 Sep;41(9):1153-60. doi: 10.1016/j.ijom.2012.03.031. Epub 2012 May 29.
When bone morphogenetic protein (BMP) is delivered to matrices in vivo may affect tissue engineered bone constructs for jaw reconstruction after cancer surgery. This study compared the effects of BMP application at different times after matrix implantation for heterotopic bone induction in a rat model. Hydroxyapatite blocks were implanted unilaterally onto the surface of the latissimus dorsi muscle. A second block was implanted onto the contralateral muscle after 1, 2 or 4 weeks and 200 μg rhBMP-2 was injected into the blocks on both sides. Bone formation and density inside the blocks was analysed by CT and histology. 8 weeks after BMP application increases in bone density within the scaffolds were most pronounced in the simultaneous application group (179 HU). Less pronounced increases were observed for the 1 (65 HU), 2 (58 HU) and 4 (31 HU; p<0.0001) week delay group. Homogeneous bone induction started from the central channel of the blocks. Capillaries and larger vessels were seen in all constructs, samples receiving delayed BMP treatment demonstrated significantly greater neovascularization. Delayed application of BMP was less effective for heterotopic bone formation than simultaneous application. A central channel allows homogeneous bone induction directly from the centre of the blocks.
当骨形态发生蛋白（BMP）在体内递送到基质中时，可能会影响用于癌症手术后颌骨重建的组织工程骨构建体。本研究比较了在大鼠模型中异位骨诱导中，基质植入后不同时间应用 BMP 的效果。将羟基磷灰石块单侧植入到背阔肌表面。1、2 或 4 周后，将第二块块植入对侧肌肉，两侧块中均注射 200μg rhBMP-2。通过 CT 和组织学分析块内的骨形成和密度。BMP 应用 8 周后，支架内骨密度的增加在同期应用组中最为明显（179HU）。1 周（65HU）、2 周（58HU）和 4 周（31HU；p<0.0001）延迟组的增加则不那么明显。从块的中央通道开始均匀地诱导骨。所有构建体中均可见毛细血管和较大的血管，接受延迟 BMP 治疗的样本显示出明显更大的新生血管化。与同期应用相比，延迟应用 BMP 对异位骨形成的效果较差。中央通道允许从块的中心直接进行均匀的骨诱导。
