Yakubu Musa T
Departments of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Androl. 2012 Nov-Dec;33(6):1199-207. doi: 10.2164/jandrol.111.016287. Epub 2012 May 31.
The present study was aimed at investigating the effects of the crude alkaloids isolated from Chromolaena odorata leaves on the hormonal and spermatogenic indices of male rats. The alkaloids obtained from C odorata leaves using standard methods were administered to male rats for 60 days at the doses of 250, 500, and 1000 mg/kg body weight. Thin-layer chromatographic analysis of the alkaloid mixture produced 8 spots, 3 of which were alkaloids with R(f) values of 0.41, 0.49, and 0.55 as confirmed by the formation of orange color and creamy precipitates with both Dragendorff and Mayer reagents, respectively. The alkaloids were represented in the extract by a yield of 20.28 g, corresponding to a percentage yield of 90.05% of the total extract of 22.52 g. The final body weights of both the control and alkaloid-treated animals increased significantly (P < .05) compared with their respective body weights before treatment. The alkaloids significantly decreased (P < .05) the testes-body weight ratio; the concentrations of testicular total protein, glycogen, sialic acid, and cholesterol; and the activities of γ-glutamyl transferase, acid phosphatase, and alkaline phosphatase. The serum luteinizing hormone and follicle-stimulating hormone levels, as well as testicular and serum testosterone levels, also decreased significantly (P < .05). There were decreases in the sperm count, motility, and density, as well as morphological changes in the sperm cells. The pH and whitish-gray color of the semen were not significantly affected. All of the doses of the alkaloids increased the total mean number of sperm cell abnormalities, with the secondary type predominating over the primary sperm cell abnormality. The alterations in the levels of the hormones and secretory and synthetic constituents of the testes and the spermatotoxic effects by the alkaloids from C odorata leaves may be due to nonavailability or deprivation of testosterone to the target organ. This lack of testosterone may have consequential effects on the reproductive process of the male rat.
本研究旨在探讨从飞机草叶中分离得到的粗生物碱对雄性大鼠激素和生精指标的影响。采用标准方法从飞机草叶中提取的生物碱，以250、500和1000 mg/kg体重的剂量给雄性大鼠连续灌胃60天。生物碱混合物的薄层色谱分析产生了8个斑点，其中3个为生物碱，其R(f)值分别为0.41、0.49和0.55，分别与碘化铋钾试剂和碘化汞钾试剂反应形成橙色和乳状沉淀得以确认。提取物中生物碱的产量为20.28 g，占总提取物22.52 g的90.05%。与处理前各自的体重相比，对照组和生物碱处理组动物的最终体重均显著增加（P < 0.05）。生物碱显著降低了睾丸与体重之比、睾丸总蛋白、糖原、唾液酸和胆固醇的浓度，以及γ-谷氨酰转移酶、酸性磷酸酶和碱性磷酸酶的活性。血清黄体生成素和促卵泡激素水平以及睾丸和血清睾酮水平也显著降低（P < 0.05）。精子数量、活力和密度均下降，精子细胞形态也发生改变。精液的pH值和灰白色未受到显著影响。所有剂量的生物碱均增加了精子细胞异常总数的平均值，其中继发性异常类型多于原发性精子细胞异常。飞机草叶生物碱对睾丸激素水平、分泌和合成成分的改变以及生精毒性作用，可能是由于靶器官无法获得或缺乏睾酮所致。睾酮的缺乏可能对雄性大鼠的生殖过程产生相应影响。
