Watcho Pierre, Meli Watio Hermine, Wankeu-Nya Modeste, Ngadjui Esther, Deeh Defo Patrick, Nkeng-Efouet Pepin Alango, Nguelefack Telesphore Benoit, Kamanyi Albert
Department of Animal Biology, Faculty of Science, Animal Physiology and Phytopharmacology Laboratory, University of Dschang, P.O. BOX 67, Dschang, Cameroon.
Department of Animal Organisms Biology, Laboratory of Animal Biology and Physiology, University of Douala, P.O. BOX 24157, Douala, Cameroon.
BMC Complement Altern Med. 2017 Jan 13;17(1):42. doi: 10.1186/s12906-016-1547-5.
Androgen deficiency is a clinical syndrome resulting from the inability of the testes to produce physiological levels of testosterone due to a disturbance occurring at one or more levels of the hypothalamic-pituitary-testicular axis. The present study was undertaken to evaluate the androgenic properties of aqueous and methanolic extracts of Ficus asperifolia on normal and castrated immature rats.
Normal rats were treated either per os with aqueous or methanolic extract of Ficus asperifolia (100 mg/kg or 500 mg/kg b.w.), distilled water (10 ml/kg b.w.), 5% Tween 80 (10 ml/kg b.w.) or subcutaneously with testosterone propionate (0.5 mg/kg b.w.). Castrated rats were treated with plant extracts (100 mg/kg b.w. or 500 mg/kg b.w.) alone or with the co-administration of plant extracts and testosterone propionate (s.c., 0.5 mg/kg b.w.) or bicalutamide (2 mg/kg b.w. per os). Animals were treated once a day during four weeks. Body weight growth and relative sexual organ weights were recorded at the end of each treatment. Some biomedical parameters were measured in the plasma (proteins, cholesterol), testes (cholesterol) and epididymis (proteins).
In normal rats, Ficus asperifolia significantly (p < 0.05) increased the relative weights of the testes and all sexual-dependent organs whereas total testicular cholesterol concentration was significantly (p < 0.05) decreased. In castrated groups, treatment with Ficus asperifolia was followed by an increase in the sexual organ weights, epididymal protein and prostatic acid phosphatase concentrations. The co-administration of testosterone and plant extracts significantly (p < 0.05) increased the weight of accessory sexual organs and epididymal protein contents. In the presence of bicalutamide (an anti-androgen), the sexual stimulating activity of Ficus asperifolia was diminished with remarkable effects on vas deferens weight (p < 0.05), plasma (p < 0.01) and epididymal (p < 0.05) protein contents.
Ficus asperifolia possesses androgen-like activity through possible stimulation of cytoplasmic and/or nuclear receptors by the bioactive compounds found in its extracts.
雄激素缺乏是一种临床综合征，由于下丘脑 - 垂体 - 睾丸轴的一个或多个水平发生紊乱，导致睾丸无法产生生理水平的睾酮。本研究旨在评估粗叶榕水提取物和甲醇提取物对正常及去势未成熟大鼠的雄激素特性。
正常大鼠经口给予粗叶榕水提取物或甲醇提取物（100mg/kg或500mg/kg体重）、蒸馏水（10ml/kg体重）、5%吐温80（10ml/kg体重），或皮下注射丙酸睾酮（0.5mg/kg体重）。去势大鼠单独给予植物提取物（100mg/kg体重或500mg/kg体重），或同时给予植物提取物与丙酸睾酮（皮下注射，0.5mg/kg体重）或比卡鲁胺（经口，2mg/kg体重）。动物连续四周每天接受一次治疗。在每次治疗结束时记录体重增长和相对性器官重量。检测血浆（蛋白质、胆固醇）、睾丸（胆固醇）和附睾（蛋白质）中的一些生物医学参数。
在正常大鼠中，粗叶榕显著（p<0.05）增加了睾丸及所有性依赖器官的相对重量，而睾丸总胆固醇浓度显著（p<0.05）降低。在去势组中，给予粗叶榕治疗后性器官重量、附睾蛋白质和前列腺酸性磷酸酶浓度增加。睾酮与植物提取物联合使用显著（p<0.05）增加了附属性器官重量和附睾蛋白质含量。在存在比卡鲁胺（一种抗雄激素）的情况下，粗叶榕的性刺激活性减弱，对输精管重量（p<0.05）、血浆（p<0.01）和附睾（p<0.05）蛋白质含量有显著影响。
粗叶榕通过其提取物中发现的生物活性化合物可能刺激细胞质和/或核受体而具有雄激素样活性。
