Yakubu M T, Akanji M A, Oladiji A T
Medicinal Plants Research Laboratory, Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
Andrologia. 2007 Dec;39(6):235-43. doi: 10.1111/j.1439-0272.2007.00792.x.
The antiandrogenic effect of oral administration of aqueous extract of Chromolaena odoratum leaves (250 and 500 mg kg(-1) body weight) for 14 days in male albino rats was investigated. Forty-two white albino rats were randomly divided into three groups: A, B and C. Group A which served as the control received 1 ml of distilled water (the vehicle) twice daily for 14 days, whereas groups B and C were treated in the same way like the control except that the animals received 250 and 500 mg kg(-1) body weight of the plant extract respectively. Compared with the control, extract administration at 250 and 500 mg kg(-1) body weight revealed a significant reduction (P<0.05) in testicular body weight ratio, acid phosphatase activities, protein, cholesterol, glycogen, sialic acid and testosterone concentrations with a significant increase (P<0.05) in lactate dehydrogenase and gamma-glutamyl transferase activities. There was no significant change (P>0.05) in serum concentrations of follicle stimulating and luteinising hormones. Histological examination revealed disruption in the arrangement of seminiferous tubules with no distinct basement membrane. These changes were accompanied by reduction in the number of spermatozoa. All these results indicated that aqueous extract of C. odoratum leaves possesses antiandrogenic property by interfering with steroidogenesis at the testicular level and this will adversely affect the functional capacity of the testes and the fertility of the animal.
研究了在雄性白化大鼠中口服臭草叶水提取物（250和500毫克/千克体重）14天的抗雄激素作用。42只白色白化大鼠随机分为三组：A组、B组和C组。作为对照组的A组每天两次接受1毫升蒸馏水（赋形剂），持续14天，而B组和C组的处理方式与对照组相同，只是动物分别接受250和500毫克/千克体重的植物提取物。与对照组相比，给予250和500毫克/千克体重提取物后，睾丸体重比、酸性磷酸酶活性、蛋白质、胆固醇、糖原、唾液酸和睾酮浓度显著降低（P<0.05），而乳酸脱氢酶和γ-谷氨酰转移酶活性显著升高（P<0.05）。促卵泡激素和促黄体生成素的血清浓度没有显著变化（P>0.05）。组织学检查显示生精小管排列紊乱，无明显基底膜。这些变化伴随着精子数量的减少。所有这些结果表明，臭草叶水提取物通过在睾丸水平干扰类固醇生成而具有抗雄激素特性，这将对睾丸的功能能力和动物的生育能力产生不利影响。
