Department of Animal Pathology, Prophylaxis and Food Hygiene, Faculty of Veterinary Medicine, University of Pisa, Italy.
Res Vet Sci. 2012 Dec;93(3):1136-8. doi: 10.1016/j.rvsc.2012.05.006. Epub 2012 May 31.
The occurrence of Mycobacterium species in clinically healthy pet reptiles was studied in Italy during the period 2004-2006. The feces samples of 223 animals were examined bacteriologically. Thirty-seven strains were isolated, in particular from 13/18 (72.2%) ophidians, 13/134 (9.7%) saurians and 11/71 (15.5%) chelonians. The isolates were classified, after HPLC analysis of bromophenacyl esters of cell wall mycolic acids, as Mycobacterium fortuitum (14 strains, 37.8%), Mycobacterium fortuitum-like (17, 45.9%), Mycobacterium peregrinum (4, 10.8%), and Mycobacterium chelonae (1, 2.7%). M. fortuitum was isolated from seven pythons, five saurians and two turtles; M. fortuitum-like from six saurians, six pythons and five turtles; M. peregrinum from four turtles; M. chelonae from one lizard. One isolate from an Iguana iguana could not be identified by HPLC analysis showing a previously unreported profile. Comparative 16S rDNA sequencing showed a low similarity with Mycobacterium triviale (97.2%) and Mycobacterium confluentis (97.1%). On the basis of such data the unidentified bacterium turned out to belong to a not yet described Mycobacterium species.
2004 年至 2006 年期间，意大利对临床健康宠物爬行动物中分枝杆菌属物种的发生情况进行了研究。对 223 只动物的粪便样本进行了细菌学检查。分离出 37 株菌株，特别是从 18 条蛇（72.2%）、13 条蜥蜴（9.7%）和 11 只龟（15.5%）中分离出来。根据细胞壁分枝菌酸溴苯甲酰酯的高效液相色谱分析，将分离株分类为偶然分枝杆菌（14 株，37.8%）、类似于偶然分枝杆菌（17 株，45.9%）、佩雷格林姆分枝杆菌（4 株，10.8%）和溃疡分枝杆菌（1 株，2.7%）。M. fortuitum 从 7 条蟒蛇、5 条蜥蜴和 2 只海龟中分离出来；类似于 M. fortuitum 从 6 条蜥蜴、6 条蟒蛇和 5 只海龟中分离出来；M. peregrinum 从 4 只海龟中分离出来；M. chelonae 从 1 只蜥蜴中分离出来。从一只鬣蜥中分离出的一株无法通过 HPLC 分析鉴定，显示出以前未报道的图谱。16S rDNA 序列比较显示与分枝杆菌（Mycobacterium triviale）（97.2%）和分枝杆菌（Mycobacterium confluentis）（97.1%）相似度较低。根据这些数据，未鉴定的细菌属于尚未描述的分枝杆菌种。
