Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):946-951. doi: 10.1099/ijs.0.043471-0. Epub 2012 Jun 1.
A carotenoid-containing chemoheterotrophic bacterium, designated IMCC1507(T), was isolated from a tidal flat sediment of the Yellow Sea, Korea. Strain IMCC1507(T) was Gram-negative, yellow, obligately aerobic, non-motile and flexirubin-negative. 16S rRNA gene sequence analysis indicated that strain IMCC1507(T) belonged to the genus Lutibacter in the family Flavobacteriaceae and exhibited 96.1-97.3 % 16S rRNA sequence similarity with the type strains of described species of the genus Lutibacter. DNA-DNA relatedness between strain IMCC1507(T) and Lutibacter litoralis KCCM 42118(T) ranged from 3.5±2.2 % to 11.2±2.4 %, indicating that strain IMCC1507(T) represented a novel genomic species in the genus Lutibacter. Chemotaxonomic characteristics of the isolate, i.e. the DNA G+C content (31.4 mol%), iso-C15 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0 as the major fatty acids, MK-6 as the predominant menaquinone and phosphatidylethanolamine as the major polar lipid, were consistent with its assignment to the genus Lutibacter. However, several phenotypic characteristics, including hydrolysis of macromolecules, enzyme activities and carbon source oxidation, differentiated strain IMCC1507(T) from members of the genus. Data from this study indicate that strain IMCC1507(T) represents a novel species in the genus Lutibacter, for which the name Lutibacter flavus sp. nov. is proposed. The type strain is IMCC1507(T) ( = KACC 14312(T) = NBRC 107589(T)).
一株含类胡萝卜素的化能异养细菌，命名为 IMCC1507(T)，从韩国黄海潮滩沉积物中分离得到。菌株 IMCC1507(T) 革兰氏阴性，黄色，严格需氧，非运动性，不含 flexirubin。16S rRNA 基因序列分析表明，菌株 IMCC1507(T) 属于黄杆菌科 Lutibacter 属，与描述的 Lutibacter 属种的模式菌株的 16S rRNA 序列相似性为 96.1-97.3%。菌株 IMCC1507(T)与 Lutibacter litoralis KCCM 42118(T)之间的 DNA-DNA 同源性为 3.5±2.2%至 11.2±2.4%，表明菌株 IMCC1507(T)代表 Lutibacter 属中的一个新型基因组种。该分离株的化学分类特征，即 DNA G+C 含量（31.4 mol%）、iso-C15：0 3-OH、iso-C15：0、iso-C15：1 G 和 anteiso-C15：0 为主要脂肪酸，MK-6 为主要的menaquinone 和磷脂酰乙醇胺为主要的极性脂，与 Lutibacter 的归属一致。然而，一些表型特征，包括大分子的水解、酶活性和碳源氧化，将菌株 IMCC1507(T)与属内的成员区分开来。本研究的数据表明，菌株 IMCC1507(T)代表 Lutibacter 属中的一个新种，建议将其命名为 Lutibacter flavus sp. nov.。模式菌株为 IMCC1507(T)（ = KACC 14312(T) = NBRC 107589(T)）。
