Marine College, Shandong University, Weihai, Shandong 264209, PR China.
Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3154-3161. doi: 10.1099/ijsem.0.004146.
A Gram-stain-negative, aerobic, non-motile, non-gliding, yellow-pigmented and rod-shaped bacterial strain, designated 1KV19, was isolated from a surface sediment sample collected near a bay in the Arctic. Growth of strain 1KV19 occurred in 1-4 % (w/v) NaCl (optimum, 2 %), at 4-35 °C (optimum, 25-30 °C) and at pH 6.5-8.0 (optimum, pH 7.0-7.5). The phylogenetic trees based on the 16S rRNA gene sequences showed that strain 1KV19 was associated with the genus and had the highest 16S rRNA gene sequence similarity to 325-5 with 98.1 % similarity. Similarity values between strain 1KV19 and the type strains of other species were in the range 95.9-97.6 %. The average nucleotide identity and digital DNA-DNA hybridization values between strain 1KV19 and related species of the genus were 76.4-79.1 and 19.9-22.3 %, respectively. The major cellular fatty acids of strain 1KV19 were iso-C 3-OH, iso-C and iso-C H. The respiratory quinone was MK-6. The major polar lipids of strain 1KV19 were phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids. The phenotypic, genotypic and chemotaxonomic differences between strain 1KV19 and its phylogenetic relatives indicate that strain 1KV19 should be regarded as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain is 1KV19 (=KCTC 62595=MCCC 1H00307).
一株革兰氏阴性、需氧、非运动、非滑行、黄色、杆状的细菌菌株，被命名为 1KV19，从北极湾附近的一个表面沉积物样本中分离得到。菌株 1KV19 在 1-4%（w/v）NaCl（最佳，2%）、4-35°C（最佳，25-30°C）和 pH 6.5-8. 0（最佳，pH 7.0-7.5）的条件下生长。基于 16S rRNA 基因序列的系统发育树显示，菌株 1KV19与属有关，与 325-5 的 16S rRNA 基因序列相似性最高，为 98.1%。菌株 1KV19 与其他属的模式菌株的相似性值在 95.9-97.6%之间。菌株 1KV19 与属内相关种的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 76.4-79.1%和 19.9-22.3%。菌株 1KV19 的主要细胞脂肪酸为 iso-C 3-OH、iso-C 和 iso-C H。呼吸醌为 MK-6。菌株 1KV19 的主要极性脂为磷脂酰乙醇胺、一种未鉴定的胺脂和两种未鉴定的极性脂。菌株 1KV19 与其系统发育上的近亲之间的表型、基因型和化学分类学差异表明，菌株 1KV19 应被视为属中的一个新种，建议将其命名为 sp. nov.。模式菌株为 1KV19（=KCTC 62595=MCCC 1H00307）。
