Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):420-424. doi: 10.1099/ijs.0.030320-0. Epub 2011 Apr 1.
A gram-negative, non-spore-forming, non-motile rod, designated MA-My1(T), was isolated from a tidal flat sediment of the South Sea, Korea. Strain MA-My1(T) grew optimally at pH 7.0-7.5, at 30 °C and with 2.0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MA-My1(T) clustered with Lutibacter litoralis CL-TF09(T) and Lutibacter maritimus S7-2(T), with which it exhibited 97.3 and 95.3 % 16S rRNA gene sequence similarity, respectively. Strain MA-My1(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(15 : 0) 3-OH and iso-C(16 : 0) 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 30.6 mol% and DNA-DNA relatedness between strain MA-My1(T) and L. litoralis JCM 13034(T) was 6.3±0.8 %. The differential phenotypic properties, together with phylogenetic and genotypic distinctiveness, distinguished strain MA-My1(T) from the members of the genus Lutibacter. On the basis of the data presented, strain MA-My1(T) is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter aestuarii sp. nov. is proposed. The type strain is MA-My1(T) ( = KCTC 23499(T) = CCUG 60022(T)).
一株革兰氏阴性、非孢子形成、非运动的棒状菌，被命名为 MA-My1(T)，从韩国南海的潮滩沉积物中分离得到。菌株 MA-My1(T)在 pH 值为 7.0-7.5、30°C 和 2.0-3.0%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MA-My1(T)与 Lutibacter litoralis CL-TF09(T)和 Lutibacter maritimus S7-2(T)聚为一簇，其 16S rRNA 基因序列相似性分别为 97.3%和 95.3%。菌株 MA-My1(T)含有 MK-6 作为主要的menaquinone，以及 iso-C(15:0)、iso-C(15:0) 3-OH 和 iso-C(16:0) 3-OH 作为主要脂肪酸。主要的极性脂为磷脂酰乙醇胺和两种未鉴定的脂。DNA G+C 含量为 30.6 mol%，菌株 MA-My1(T)与 Lutibacter litoralis JCM 13034(T)之间的 DNA-DNA 同源性为 6.3±0.8%。表型特征、系统发育和基因型的差异将菌株 MA-My1(T)与 Lutibacter 属的其他成员区分开来。基于所提供的数据，菌株 MA-My1(T)被认为代表 Lutibacter 属的一个新种，提议将其命名为 Lutibacter aestuarii sp. nov.。其模式菌株为 MA-My1(T)（=KCTC 23499(T)=CCUG 60022(T)）。
