Greywoode G I, McCarthy S P, McGee J O
University of Oxford, Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital.
J Clin Pathol. 1990 Dec;43(12):992-6. doi: 10.1136/jcp.43.12.992.
When first reported, EBM/11 reacted with mononuclear phagocyte system cells only in fresh frozen sections, but it has been shown to have similar cellular specificity in routine formalin fixed, paraffin wax embedded tissue. This was achieved by limited proteolysis with protease XIV before immunocytochemical staining. In archival biopsy specimens EBM/11 produced granular cytoplasmic staining of alveolar macrophages, Kupffer cells, tingible body macrophages and sinus histiocytes, cells of splenic cords, cortical and medullary macrophages of thymus; blood monocytes, peritoneal and mesothelial macrophages; bone marrow mononuclear cells, megakaryocytes and osteoclasts; lamina propria macrophages in the gastrointestinal tract, and connective tissue cells (presumptive macrophages) of thyroid, gall bladder, skin, pancreas, ovary, myometrium, endometrium, cervix, kidney, prostate, placenta, myocardium and breast. Unlike other anti-macrophage antibodies, EBM/11 did not react with granulocytes, lymphocytes, plasma cells, platelets, endothelial and epithelial cells in paraffin wax sections. It did not label skin Langerhans' cells, microglial cells, and interdigitating reticulum cells (as in frozen sections). This study opens a new area for the specific identification by EBM/11 of mononuclear phagocyte system cells in archival biopsy specimens. It also raises the possibility that some monoclonal antibodies, believed to be reactive only in frozen sections, may react in archival tissue after limited proteolysis with an appropriate enzyme.
首次报道时，EBM/11仅在新鲜冰冻切片中与单核吞噬细胞系统细胞发生反应，但已证明它在常规福尔马林固定、石蜡包埋组织中具有相似的细胞特异性。这是通过在免疫细胞化学染色前用蛋白酶XIV进行有限的蛋白水解来实现的。在存档活检标本中，EBM/11在肺泡巨噬细胞、库普弗细胞、吞噬体巨噬细胞和窦组织细胞、脾索细胞、胸腺皮质和髓质巨噬细胞中产生颗粒状细胞质染色；血液单核细胞、腹膜和间皮巨噬细胞；骨髓单核细胞、巨核细胞和破骨细胞；胃肠道固有层巨噬细胞，以及甲状腺、胆囊、皮肤、胰腺、卵巢、子宫肌层、子宫内膜、宫颈、肾脏、前列腺、胎盘、心肌和乳腺的结缔组织细胞（推测为巨噬细胞）。与其他抗巨噬细胞抗体不同，EBM/11在石蜡切片中不与粒细胞、淋巴细胞、浆细胞、血小板、内皮细胞和上皮细胞发生反应。它不标记皮肤朗格汉斯细胞、小胶质细胞和交错突网状细胞（如在冰冻切片中）。这项研究为EBM/11在存档活检标本中特异性识别单核吞噬细胞系统细胞开辟了一个新领域。它还提出了一种可能性，即一些被认为仅在冰冻切片中具有反应性的单克隆抗体，在用适当的酶进行有限的蛋白水解后，可能在存档组织中发生反应。
