评估一种用于检测溶组织内阿米巴的即时粪便抗原检测试验。
Evaluation of a rapid point-of-care fecal antigen detection test for Entamoeba histolytica.
机构信息
Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA 22908-1337, USA.
出版信息
Am J Trop Med Hyg. 2012 Jun;86(6):980-1. doi: 10.4269/ajtmh.2012.11-0661.
Abstract
Amebiasis is a major cause of morbidity and mortality in the developing world. A reliable point-of-care test would help to improve diagnosis and early treatment. We evaluated a novel rapid fecal antigen detection test for E. histolytica (E. HISTOLYTICA QUIK CHEK; TechLab, Inc., Blacksburg, VA), in a cohort of children in Bangladesh where amebiasis is endemic. This point-of-care test had a sensitivity of 100% and a specificity of 100% when compared with enzyme-linked immunosorbent assay antigen detection.
摘要
阿米巴病是发展中国家发病率和死亡率的主要原因。一种可靠的即时检测方法将有助于改善诊断和早期治疗。我们在孟加拉国的一个阿米巴病流行的儿童队列中评估了一种新的快速粪便抗原检测技术（TechLab，弗吉尼亚州布莱克斯堡的 E. histolytica [E. HISTOLYTICA QUIK CHEK]）。与酶联免疫吸附试验抗原检测相比，该即时检测方法的敏感性为 100%，特异性为 100%。
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1
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2
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J Clin Microbiol. 2005 May;43(5):2168-72. doi: 10.1128/JCM.43.5.2168-2172.2005.
3
Amebiasis.阿米巴病
N Engl J Med. 2003 Apr 17;348(16):1565-73. doi: 10.1056/NEJMra022710.
4
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5
Entamoeba histolytica and Entamoeba dispar: epidemiology and comparison of diagnostic methods in a setting of nonendemicity.溶组织内阿米巴和迪斯帕内阿米巴：非流行地区的流行病学及诊断方法比较
Clin Infect Dis. 1999 Nov;29(5):1315-8. doi: 10.1086/313433.
6
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Am J Trop Med Hyg. 1994 Apr;50(4):412-9. doi: 10.4269/ajtmh.1994.50.412.
7
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8
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J Immunol. 1990 Jun 15;144(12):4803-9.
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