Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sensors (Basel). 2012;12(4):4065-73. doi: 10.3390/s120404065. Epub 2012 Mar 27.
An N-acylhomoserine lactone (AHL)-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v). L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya sauce liquid state fermentation.
从酱油发酵卤水中分离到一株能够降解 N-酰基高丝氨酸内酯（AHL）的细菌 L62，采用富含酱油（10%v/v）的琼脂培养基进行分离。L62 是一株具有淀粉水解活性的杆状革兰氏阳性菌，16S rDNA 和 rpoB 序列分析表明其与解淀粉芽孢杆菌亲缘关系最近。B. sonorensis L62 能够高效降解 N-3-氧代己酰高丝氨酸内酯和 N-辛酰高丝氨酸内酯。然而，并未在 L62 中检测到编码 AHL 降解酶的aiiA 同源物，这表明 L62 中存在不同的 AHL 降解基因。据我们所知，这是首次从酱油液态发酵中分离到能够降解 AHL 的解淀粉芽孢杆菌。
