Urita Yoshihisa, Watanabe Toshiyasu, Imai Tsunehiko, Miura Yasuyuki, Honda Yoshiko, Washizawa Naohiro, Sanaka Masaki, Shimada Nagato, Nakajima Hitoshi, Sugimoto Motonobu
Department of General Medicine and Emergency Care, Toho University School of Medicine, Tokyo, Japan.
N Am J Med Sci. 2009 Oct;1(5):239-43. doi: 10.4297/najms.2009.5239.
The usefulness of the typical direct methods involving duodenal intubation, such as the secretin and secretin-cholecystokinin tests, in the diagnosis of exocrine pancreatic dysfunction is widely accepted. However, these diagnostic tests tend to be avoided because of their technical complexity and the burden on patients. Recently, a simple breath test was developed for assessment of exocrine pancreatic function employing 13C-dipeptide [i.e., benzoyl-L-tyrosyl-[1-13C] alanine (Bz-Tyr-Ala)]. Although alcohol abuse causes pancreatic damage in humans, this has been unclear in rats.
The aim of the study is to evaluate the effect of ethanol exposure beginning at an early age on extra-pancreatic secretory function in rats.
Twelve female rats of the F344 strain aged 12 months were used. Seven rats were fed on a commercial mash food with 16% ethanol solution (Japanese Sake) as drinking-fluid since at 29 days of age (ethanol group). The remaining five rats were fed on a nutrient-matched isocaloric diet with water as drinking-fluid (control group). After 24-hr fasting, rats are orally administrated 1cc of water containing sodium 13C-dipeptide (5 mg/kg) and housed in an animal chamber. The expired air in the chamber is collected in a breath-sampling bag using a tube and aspiration pump. The 13CO2 concentration is measured using an infrared spectrometer at 10-min interval for 120 min and expressed as delta per mil.
The breath 13CO2 level increased and peaked at 20 min in both two groups. In general, 13CO2 excretion peaked rapidly and also decreased sooner in ethanol rats than in control rats. The mean value of the maximal 13CO2 excretion is 34.7 per mil in ethanol rats, greater than in control rats (31.4 per mil), but the difference did not reach the statistically significance.
Chronic ethanol feeding beginning at an early age does not affect extra-pancreatic secretory function in rats.
十二指肠插管的典型直接方法，如促胰液素和促胰液素-胆囊收缩素试验，在诊断外分泌性胰腺功能障碍中的作用已被广泛认可。然而，由于技术复杂性和给患者带来的负担，这些诊断测试往往不被采用。最近，开发了一种简单的呼气试验，使用13C-二肽[即苯甲酰-L-酪氨酰-[1-13C]丙氨酸（Bz-Tyr-Ala）]来评估外分泌性胰腺功能。虽然酒精滥用会导致人类胰腺损伤，但在大鼠中情况尚不清楚。
本研究的目的是评估幼年开始乙醇暴露对大鼠胰腺外分泌功能的影响。
使用12只12月龄的F344品系雌性大鼠。7只大鼠自29日龄起以含16%乙醇溶液（日本清酒）的市售混合饲料为食并以此作为饮用水（乙醇组）。其余5只大鼠以营养匹配的等热量饮食为食并以水作为饮用水（对照组）。禁食24小时后，给大鼠口服1毫升含13C-二肽钠（5毫克/千克）的水，并置于动物饲养室。饲养室内的呼出气体通过管子和抽吸泵收集到呼气采样袋中。使用红外光谱仪每隔10分钟测量13CO2浓度，持续120分钟，并以千分比表示。
两组大鼠呼出气体中的13CO2水平均升高，并在20分钟时达到峰值。一般来说，乙醇组大鼠的13CO2排泄峰值出现得更快，下降也比对照组大鼠更早。乙醇组大鼠最大13CO2排泄的平均值为34.‰，高于对照组大鼠（31.4‰），但差异未达到统计学意义。
幼年开始的慢性乙醇喂养不影响大鼠的胰腺外分泌功能。
