Zhang Meili, Ni Peihua, Cai Changping, Chen Nijun, Wang Shili
Department of Otorhinolaryngology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Mar;26(5):197-200, 204. doi: 10.13201/j.issn.1001-1781.2012.05.006.
To investigate the relationship between the promoter polymorphism of IL6 (-174G > C, -572G > C and -597G > A) and chronic rhinosinusitis (CRS).
The case-control study consisted of 123 patients with CRS and 239 controls from a Chinese Han population from Shanghai. The genotypes of the subjects were determined by polymerase chain reaction-restriction fragment length polymorphism and gene sequencing. Besides, the concentrations of the totle immunoglobulin E (TIgE) and eosinophilic cationic protein (ECP) in the blood were also determined.
The -174G > C and -597G > A polymorphisms were not detected in this study population. Significant differences in genotype and allele frequencies of -572C/G were observed between CRS patients and control groups. In CRS patients, the CC, CG, GG genotype frequencies were 69.1%, 29.3%, 1.6%, C, G allele frequencies were 83.7%, 16.3%. In control group, the genotype frequencies were 55.2%, 42.3%, 2.5%, the allele frequencies were 76.4%, 23.6%, respectively. The -572CC genotype was associated with an increased risk of developing CRS (P < 0.05, OR = 1.932, 95% CI, 1.205-3.097). There was no significant differences in the concentrations of the TIgE and ECP among each genotype.
IL-6 gene -572G > C polymorphism is associated with the susceptibility to CRS. CC genotype could be an independent risk factor.
探讨白细胞介素6（IL6）启动子多态性（-174G＞C、-572G＞C和-597G＞A）与慢性鼻-鼻窦炎（CRS）之间的关系。
病例对照研究纳入了123例CRS患者和239名来自上海的中国汉族对照。采用聚合酶链反应-限制性片段长度多态性和基因测序法确定研究对象的基因型。此外，还检测了血液中总免疫球蛋白E（TIgE）和嗜酸性粒细胞阳离子蛋白（ECP）的浓度。
本研究人群未检测到-174G＞C和-597G＞A多态性。CRS患者与对照组之间-572C/G的基因型和等位基因频率存在显著差异。CRS患者中，CC、CG、GG基因型频率分别为69.1%、29.3%、1.6%，C、G等位基因频率分别为83.7%、16.3%。对照组中，基因型频率分别为55.2%、42.3%、2.5%，等位基因频率分别为76.4%、23.6%。-572CC基因型与CRS发病风险增加相关（P＜0.05，OR=1.932，95%CI，1.205-3.097）。各基因型间TIgE和ECP浓度无显著差异。
IL-6基因-572G＞C多态性与CRS易感性相关。CC基因型可能是一个独立的危险因素。
