Ghasemi Hassan, Owlia Parviz, Ghazanfari Tooba, Yaraee Roya, Saderi Horieh, Soroush Mohammad Reza, Naghizadeh Mohammad Mehdi
Department of Ophthalmology, Shahed University, Tehran, Iran.
Cutan Ocul Toxicol. 2013 Mar;32(1):13-7. doi: 10.3109/15569527.2012.692136. Epub 2012 Jun 6.
Ocular surface disorders and infections in sulfur mustard (SM) exposed patients are of particular clinical importance. The aim of the present study is to detect the conjunctival bacterial florae in patients with seriously SM induced eye injuries.
Conjunctival bacterial florae of 143 seriously eye injured subjects as the study group was detected. The results were compared with 26 normal participants. Both groups were matched in age and sex. The samples were taken by sterile swab from interior fornixes of conjunctiva in both groups and were transported to microbiology laboratory by Stuart's Transport Medium. All samples were inoculated onto Blood agar, Mac Conkey agar and Chocolate agar and isolated microorganisms were identified by biochemical tests. The data were analyzed by SPSS and Man Whitney tests.
Nineteen cases (13.39%) and none of the controls (0%) had positive culture results (p = .043). Isolated microorganisms from patients included coagulase-negative staphylococci 10 cases (52.6%), Staphylococcus aureus 5 cases (26.3%), non enterobacteriaceae gram negative bacilli 2 cases (10.5%), Penicillium spp. 2 cases (10.5%), Citrobacter sp. 1 case (5.2%), non-spore forming Gram positive bacillus 1 case (5.2%) and α hemolytic streptococcus 1 case (5.2%). Two patients had mixed microorganisms and other patients had just one microorganism. Most of the S. aureus isolates were sensitive to usual antibiotics.
The results of this study showed that the prevalence rate of conjunctival bacterial isolates in patients with seriously SM induced ocular injuries are higher and potentially more dangerous than normal controls.
硫芥（SM）暴露患者的眼表疾病和感染具有特殊的临床重要性。本研究的目的是检测严重硫芥致眼损伤患者的结膜细菌菌群。
检测了143例严重眼损伤受试者作为研究组的结膜细菌菌群。将结果与26名正常参与者进行比较。两组在年龄和性别上相匹配。两组均用无菌拭子从结膜内穹窿取材，并通过Stuart运送培养基送至微生物实验室。所有样本接种于血琼脂、麦康凯琼脂和巧克力琼脂上，分离出的微生物通过生化试验进行鉴定。数据采用SPSS和曼-惠特尼检验进行分析。
19例（13.39%）患者培养结果为阳性，而对照组无一例阳性（0%）（p = 0.043）。从患者中分离出的微生物包括凝固酶阴性葡萄球菌10例（52.6%）、金黄色葡萄球菌5例（26.3%）、非肠杆菌科革兰阴性杆菌2例（10.5%）、青霉属2例（10.5%）、柠檬酸杆菌属1例（5.2%）、非芽孢形成革兰阳性杆菌1例（5.2%）和α溶血性链球菌1例（5.2%）。2例患者有混合微生物，其他患者只有一种微生物。大多数金黄色葡萄球菌分离株对常用抗生素敏感。
本研究结果表明，严重硫芥致眼损伤患者结膜细菌分离株的患病率高于正常对照组，且潜在危险性更大。
