银纳米粒子通过 Toll 样受体 2 途径诱导细胞凋亡。
Silver nanoparticles induce apoptosis through the toll-like receptor 2 pathway.
机构信息
Department of Dentistry, Hallym University, Kyounggi-do, Korea.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Jun;113(6):789-98. doi: 10.1016/j.oooo.2012.01.019.
OBJECTIVE
The objective of this study was to evaluate the effects of silver nanoparticles (AgNPs) on the Toll-like receptor 2 (TLR-2) pathway in cultured cells.
STUDY DESIGN
Human chondrocytes, periodontal ligament (PDL) cells, and SCC-9 cells (squamous cell carcinoma from the tongue) were cultured and subjected to cytotoxicity assays. To evaluate the effects of AgNPs on the TLR-2 pathway, TLR-2 small interfering (si) RNA or TLR-2 antibodies were applied to the chondrocytes, followed by the application of AgNPs.
RESULTS
AgNPs induced dose-dependent effects on the examined cell types in terms of both cytotoxicity and TLR-2 expression levels. AgNP-mediated apoptosis was reduced after treatment with TLR-2 siRNA in both PDL cells and chondrocytes. Furthermore, functional blocking of TLR-2 with anti-TLR2 antibodies inhibited AgNP-mediated cytotoxicity. AgNPs increased c-Jun phosphorylation, an effect that was reversed after treatment with TLR-2 siRNA.
CONCLUSIONS
The results indicate that AgNP-mediated apoptosis most likely occurs via the TLR-2 pathway.
目的
本研究旨在评估纳米银颗粒（AgNPs）对培养细胞中 Toll 样受体 2（TLR-2）通路的影响。
研究设计
培养人软骨细胞、牙周膜（PDL）细胞和 SCC-9 细胞（舌鳞癌细胞），并进行细胞毒性测定。为了评估 AgNPs 对 TLR-2 通路的影响，将 TLR-2 小干扰（si）RNA 或 TLR-2 抗体应用于软骨细胞，然后再应用 AgNPs。
结果
AgNPs 对所检测的细胞类型均表现出剂量依赖性的细胞毒性和 TLR-2 表达水平的影响。在 PDL 细胞和软骨细胞中，用 TLR-2 siRNA 处理后，AgNP 介导的细胞凋亡减少。此外，用抗 TLR2 抗体阻断 TLR-2 的功能可抑制 AgNP 介导的细胞毒性。AgNPs 增加 c-Jun 磷酸化，用 TLR-2 siRNA 处理后可逆转这一作用。
结论
结果表明，AgNP 介导的细胞凋亡很可能是通过 TLR-2 通路发生的。
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