Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27606, USA.
J Mater Sci Mater Med. 2012 Sep;23(9):2115-26. doi: 10.1007/s10856-012-4691-6. Epub 2012 Jun 5.
Hydroxyapatite-gelatin modified siloxane (GEMOSIL) nanocomposite was developed by coating, kneading and hardening processes to provide formable scaffolding for alloplastic graft applications. The present study aims to characterize scaffolding formability and mechanical properties of GEMOSIL, and to test the in vitro and in vivo biocompatibility of GEMOSIL. Buffer Solution initiated formable paste followed by the sol-gel reaction led to a final hardened composite. Results showed the adequate coating of aminosilane, 11-19 wt%, affected the cohesiveness of the powders and the final compressive strength (69 MPa) of the composite. TGA and TEM results showed the effective aminosilane coating that preserves hydroxyapatite-gelatin nanocrystals from damage. Both GEMOSIL with and without titania increased the mineralization of preosteoblasts in vitro. Only did titania additives revealed good in vivo bone formation in rat calvarium defects. The scaffolding formability, due to cohesive bonding among GEMOSIL particles, could be further refined to fulfill the complicated scaffold processes.
羟基磷灰石-明胶改性硅氧烷（GEMOSIL）纳米复合材料通过涂层、揉捏和硬化工艺制备，为同种异体移植物应用提供可成型的支架。本研究旨在表征 GEMOSIL 的支架成型性和机械性能，并测试 GEMOSIL 的体外和体内生物相容性。缓冲溶液引发可成型糊剂，随后发生溶胶-凝胶反应，最终得到硬化复合材料。结果表明，氨基硅烷的适当涂层（11-19wt%）影响了粉末的内聚性和复合材料的最终抗压强度（69MPa）。TGA 和 TEM 结果表明，氨基硅烷的有效涂层可防止羟基磷灰石-明胶纳米晶体受损。含有和不含有二氧化钛的 GEMOSIL 都能增加体外成骨前体细胞的矿化。只有二氧化钛添加剂才能在大鼠颅骨缺损中显示出良好的体内骨形成。由于 GEMOSIL 颗粒之间的粘性结合，支架成型性可以进一步细化，以满足复杂的支架工艺。
