Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, Henan, China.
Anal Bioanal Chem. 2020 Sep;412(23):5811-5817. doi: 10.1007/s00216-020-02803-5. Epub 2020 Jul 11.
Using the surface molecular imprinting technique, a thermo-sensitive molecularly imprinted fluorescent sensor was constructed for bovine hemoglobin (BHb) detection with the silanized carbon dots (CD@SiO) as fluorescent signal, N-isopropylacrylamide as monomer sensitive to temperature, and BHb as template. The silanized carbon dots coated by the molecularly imprinted polymer (CD@SiO@MIP) were characterized by high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and fluorescence spectroscopy. Owing to the combination of the strong fluorescence sensitivity of CDs and the high selectivity of the molecular imprinting shell, the prepared sensor showed good recognition and detection performance to the target protein BHb, with a linear range of 0.31-1.55 μM and a detection limit of 1.55 μM. Furthermore, the sensor was utilized to detect the content of BHb in real urine with a recovery of 98.6-100.5%. The CD@SiO@MIP sensors present a high potential for applications in the detection of BHb in biological systems. Graphical abstract.
采用表面分子印迹技术，以硅烷化碳点（CD@SiO）作为荧光信号，以 N-异丙基丙烯酰胺作为温度敏感单体，以牛血红蛋白（BHb）为模板，构建了用于检测牛血红蛋白（BHb）的热敏分子印迹荧光传感器。通过高分辨率透射电子显微镜、傅里叶变换红外光谱和荧光光谱对涂覆有分子印迹聚合物的硅烷化碳点（CD@SiO@MIP）进行了表征。由于 CD 的强荧光灵敏度和分子印迹壳的高选择性的结合，所制备的传感器对目标蛋白 BHb 表现出良好的识别和检测性能，线性范围为 0.31-1.55 μM，检测限为 1.55 μM。此外，该传感器还用于检测实际尿液中 BHb 的含量，回收率为 98.6-100.5%。CD@SiO@MIP 传感器在生物体系中检测 BHb 方面具有很高的应用潜力。
