Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Andalas, 25163 Limau Manis, Padang, Indonesia.
Bioorg Med Chem Lett. 2012 Jul 1;22(13):4242-5. doi: 10.1016/j.bmcl.2012.05.043. Epub 2012 May 17.
A new ring A-seco triterpenoid, sentulic acid, along with a known oleanane-type triterpenoid, 3-oxoolean-12-en-27-oic acid, were isolated from the Indonesian plant Sandoricum koetjape Merr. Their chemical structures were elucidated by spectroscopic analysis. In addition, the cytotoxic effects of these compounds on human promyelocytic leukemia HL-60 cells were studied. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and trypan blue dye exclusion tests showed that sentulic acid and 3-oxoolean-12-en-27-oic acid were able to induce cytotoxicity in these cells. Furthermore, morphological examination and DNA fragmentation analysis indicated that these cytotoxic effects were mediated by apoptosis.
从印度尼西亚植物 Sandoricum koetjape Merr. 中分离得到了一种新的五环 A-降三萜，sentulic 酸，以及一种已知的齐墩果烷型三萜，3-氧代齐墩果酸。通过光谱分析阐明了它们的化学结构。此外，还研究了这些化合物对人早幼粒细胞白血病 HL-60 细胞的细胞毒性作用。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定和台盼蓝排斥试验的结果表明，sentulic 酸和 3-氧代齐墩果酸能够诱导这些细胞的细胞毒性。此外，形态学检查和 DNA 片段化分析表明，这些细胞毒性作用是通过细胞凋亡介导的。
