Baâtour Olfa, Mahmoudi Hela, Tarchoun Imen, Nasri Nawel, Trabelsi Najla, Kaddour Rym, Zaghdoudi Maha, Hamdawi Ghaith, Ksouri Riadh, Lachaâl Mokhtar, Marzouk Brahim
Unité de Physiologie et Biochimie de la Tolérance au Sel des Plantes, Département de Biologie, Faculté des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj-Cedria, 2050 Hammam-lif, Tunisia.
J Sci Food Agric. 2013 Jan 15;93(1):134-41. doi: 10.1002/jsfa.5740. Epub 2012 Jun 7.
Two varieties of Origanum majorana (Canadian and Tunisian) were evaluated for their phenolic, flavonoid and tannin contents, individual phenolic compounds and antioxidant activities under NaCl constraint.
The results showed a significant variability in phenolic composition and antioxidant behavior between the two varieties under salt stress. The phenolic composition of methanolic extracts was determined by reversed-phase high-performance liquid chromatography. Amentoflavone was the predominant flavonoid compound; in addition, trans-2-hydrocinnamic acid became the major phenolic acid with salt treatment of the Tunisian variety. In the control, Canadian variety extract was characterized by high levels of gallic acid and amentoflavone. However, under 75 mmol L(-1) NaCl, gallic acid content doubled, whereas amentoflavone content was maintained in the Canadian variety. Stimulation of phenolic acid biosynthesis was observed in these two varieties under salt treatment despite the fact that shoots of the Tunisian variety showed higher antioxidant activities compared to those from the Canadian variety. Tunisian O. majorana might have developed tolerance to salinity and avoided tissue damage by activating enzymes involved in the galactosylation of quercetin into quercetin-3-galactoside and quercetin-3-rhamnoside.
Our results confirmed the tolerance of Tunisian O. majorana plants.
对两种马郁兰（加拿大品种和突尼斯品种）在NaCl胁迫下的酚类、黄酮类和单宁含量、单个酚类化合物及抗氧化活性进行了评估。
结果表明，在盐胁迫下，两个品种之间的酚类成分和抗氧化行为存在显著差异。采用反相高效液相色谱法测定了甲醇提取物的酚类成分。穗花杉双黄酮是主要的黄酮类化合物；此外，经盐处理的突尼斯品种中，反式-2-肉桂酸成为主要的酚酸。在对照中，加拿大品种提取物的特征是含有高水平的没食子酸和穗花杉双黄酮。然而，在75 mmol L(-1) NaCl处理下，加拿大品种中没食子酸含量翻倍，而穗花杉双黄酮含量保持不变。尽管突尼斯品种的嫩枝比加拿大品种的嫩枝表现出更高的抗氧化活性，但在盐处理下，这两个品种都观察到酚酸生物合成受到刺激。突尼斯马郁兰可能通过激活参与将槲皮素半乳糖基化为槲皮素-3-半乳糖苷和槲皮素-3-鼠李糖苷的酶，从而对盐分产生耐受性并避免组织损伤。
我们的结果证实了突尼斯马郁兰植物的耐受性。
