Rowe Aaron A, White Ryan J, Bonham Andrew J, Plaxco Kevin W
Department of Chemistry and Biochemistry, University Of California Santa Barbara, USA.
J Vis Exp. 2011 Jun 1(52):2922. doi: 10.3791/2922.
As medicine is currently practiced, doctors send specimens to a central laboratory for testing and thus must wait hours or days to receive the results. Many patients would be better served by rapid, bedside tests. To this end our laboratory and others have developed a versatile, reagentless biosensor platform that supports the quantitative, reagentless, electrochemical detection of nucleic acids (DNA, RNA), proteins (including antibodies) and small molecules analytes directly in unprocessed clinical and environmental samples. In this video, we demonstrate the preparation and use of several biosensors in this "E-DNA" class. In particular, we fabricate and demonstrate sensors for the detection of a target DNA sequence in a polymerase chain reaction mixture, an HIV-specific antibody and the drug cocaine. The preparation procedure requires only three hours of hands-on effort followed by an overnight incubation, and their use requires only minutes.
在当前的医学实践中，医生将样本送到中央实验室进行检测，因此必须等待数小时或数天才能得到结果。许多患者会从快速的床边检测中受益更多。为此，我们实验室和其他实验室开发了一种多功能、无需试剂的生物传感器平台，该平台支持直接在未经处理的临床和环境样本中对核酸（DNA、RNA）、蛋白质（包括抗体）和小分子分析物进行定量、无需试剂的电化学检测。在本视频中，我们展示了几种此类“E-DNA”生物传感器的制备和使用。特别地，我们制造并展示了用于检测聚合酶链反应混合物中的目标DNA序列、HIV特异性抗体和药物可卡因的传感器。制备过程仅需三小时的实际操作时间，之后进行过夜孵育，而使用这些传感器仅需几分钟。
