Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China.
Anim Reprod Sci. 2012 Jun;132(3-4):187-92. doi: 10.1016/j.anireprosci.2012.05.002. Epub 2012 May 24.
The objectives of this study were to investigate the potential mechanisms of dietary arginine (Arg) and N-carbamoylglutamate (NCG) supplementation on reproductive performance of sows. Twenty-seven crossbred (Landrace×Large White) sows with similar body weight and parity at day (90±1) of gestation were assigned randomly into 3 groups (n=9) control group, Arg group, NCG group, and fed with the following diets: a control diet, and the control diet supplemented with 1.0% Arg or 0.1% NCG. Litter size was recorded. Blood samples were obtained for biochemical analyses. Placenta chorioallantoic membrane tissue collected immediately after birth to preserve in RNA stabilizer for mRNA analysis of endothelial nitric oxide synthase (eNOS), endothelial growth factor a (VEGFA) and placenta growth factor 1 (PlGF1) by real time-PCR. The results showed that compared with the control group, the average birth weight of all piglets born alive were 16.2% and 14.3% higher in the Arg and NCG groups (P<0.05), respectively; plasma VEGFA was higher in the Arg group (P<0.05). The expression of VEGFA in the allantochorion tissue of the NCG-supplemented group was higher (P<0.01), and tended to be higher in the Arg-supplemented group (0.05<P<0.1). NCG significantly increased protein concentration of PlGF1 in plasma (P<0.05), and enhanced PlGF1mRNA expression in allantochorion tissue of placenta (P<0.01). The results suggested that dietary Arg and NCG supplementation play important roles in meliorating placental vascular function and promoting the nutrients supply to fetus.
本研究旨在探究日粮精氨酸（Arg）和 N- 氨甲酰谷氨酸（NCG）补充对母猪繁殖性能的潜在作用机制。选择 27 头体重和胎次相近的杂交（长白猪×大白猪）母猪，于妊娠第 90±1 天随机分为 3 组（n=9）：对照组、Arg 组和 NCG 组，分别饲喂基础日粮和添加 1.0%Arg 或 0.1%NCG 的基础日粮。记录窝产仔数。采集血液样本进行生化分析。采集胎盘绒毛尿囊膜组织，立即置于 RNA 稳定剂中，用于实时 PCR 分析内皮型一氧化氮合酶（eNOS）、血管内皮生长因子 A（VEGFA）和胎盘生长因子 1（PlGF1）mRNA。结果表明：与对照组相比，Arg 组和 NCG 组所有活产仔的平均初生重分别提高了 16.2%和 14.3%（P<0.05）；Arg 组血浆 VEGFA 水平升高（P<0.05）。NCG 组绒毛尿囊膜组织中 VEGFA 的表达升高（P<0.01），Arg 组的表达也有升高的趋势（0.05<P<0.1）。NCG 显著提高了血浆中 PlGF1 蛋白浓度（P<0.05），并增强了胎盘绒毛尿囊膜组织中 PlGF1mRNA 的表达（P<0.01）。结果表明，日粮补充 Arg 和 NCG 对改善胎盘血管功能和促进胎儿营养供应具有重要作用。
