Hovaneț Marilena-Viorica, Ozon Emma Adriana, Moroșan Elena, Șeremet Oana Cristina, Oprea Eliza, Geană Elisabeta-Irina, Anghel Adriana Iuliana, Bădiceanu Carmellina, Duțu Ligia Elena, Stoicescu Cristina Silvia, Nagoda Eugenia, Ancuceanu Robert
Department of Pharmaceutical Botany and Cell Biology, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
Life (Basel). 2022 Nov 22;12(12):1947. doi: 10.3390/life12121947.
Mill. (jujube) is a well-known medicinal plant with pronounced wound healing properties. The present study aimed to establish the chemical composition of the lyophilized ethanolic extract from Romanian leaves and to evaluate the healing and anti-inflammatory properties of a newly developed lipophilic ointment containing 10% dried jujube leaves extract. The ultra-High-Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry method was used, and 47 compounds were detected, among them the novel epicatechin and caffeic acid. The extract contains significant amounts of rutin (29.836 mg/g), quercetin (15.180 mg/g) and chlorogenic acid (350.96 µg/g). The lipophilic ointment has a slightly tolerable pH, between 5.41-5.42, and proved to be non-toxic in acute dermal irritation tests on New Zealand albino rabbits and after repeated administration on Wistar rats. The ointment also has a healing activity comparable to Cicatrizin (a pharmaceutical marketed product) on Wistar rats and a moderate anti-inflammatory action compared to the control group, but statistically insignificant compared to indomethacin in the rat-induced inflammation test by intraplantar administration of kaolin. The healing and anti-inflammatory properties of the tested ointment are due to phenolic acids and flavonoids content, less because of minor components as apocynin, scopoletin, and isofraxidin.
酸枣是一种著名的药用植物，具有显著的伤口愈合特性。本研究旨在确定罗马尼亚酸枣叶冻干乙醇提取物的化学成分，并评估一种新开发的含10%干酸枣叶提取物的亲脂性软膏的愈合和抗炎特性。采用超高效液相色谱电喷雾电离串联质谱法，检测到47种化合物，其中包括新发现的表儿茶素和咖啡酸。提取物含有大量的芦丁（29.836毫克/克）、槲皮素（15.180毫克/克）和绿原酸（350.96微克/克）。亲脂性软膏的pH值在5.41-5.42之间，略有可耐受性，在对新西兰白化兔进行的急性皮肤刺激性试验以及对Wistar大鼠反复给药后，证明无毒。该软膏在Wistar大鼠身上的愈合活性与Cicatrizin（一种市售药品）相当，与对照组相比具有中等抗炎作用，但在大鼠足跖注射高岭土诱导炎症试验中，与吲哚美辛相比，统计学上无显著差异。受试软膏的愈合和抗炎特性归因于酚酸和黄酮类化合物的含量，而非诸如穿心莲内酯、东莨菪内酯和异秦皮啶等次要成分。
