Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
J Ethnopharmacol. 2012 Aug 1;142(3):789-94. doi: 10.1016/j.jep.2012.05.065. Epub 2012 Jun 7.
The rhizoma of Paris polyphylla var. yannanensis or P. polyphylla var. chinensis called Rhizoma Paridis as a traditional Chinese medicine has an effect of heat-clearing and detoxicating, detumescence and acesodyne in folk for a long time. The increasing application of Rhizoma Paridis resulted in the shortage of wildlife resources. Here, we compared the major activities of other species of genus Paris to find the replacement plants.
Six species (P. polyphylla var. yunnanensis, P. delavayi var. delavayi, P. fargesii var. Fargesii, P. bashanensis Wang et Tang, P. polyphyllar var. minora, and P. polyphylla var. pseudothibetical) were collected from three Provinces in China, and compared the hemostatic, cytotoxic and hemolytic activities by different assays.
For the hemostatic activity, all the plants except Paris fargesii var. Fargesii could significantly shorten the tail bleeding time and blood clotting time (P<0.05). For further mechanism study, they reduced the prothrombin time (PT) and activated partial thromboplastin time (APTT), but they had no significant effect on thrombin time (TT). P. fargesii var. Fargesii showed the similar cytotoxicity to P. polyphylla var. yunnanensis (IC(50): 18.21 and 15.73 μg/mL, respectively). HD(50) was used as the index of hemolytic activity. P. delavayi var. delavayi and P. bashanensis Wang et Tang were the last to have this activity as the values were 3.027 and 1.222 mg/mL.
The different species of genus Paris have different activities. Paris delavayi var. delavayi and Paris bashanensis Wang et Tang could be used as the resources of hemostatic drugs and P. fargesii var. Fargesii as the antitumor medicine.
云南重楼或中国重楼的根茎，作为一种传统中药，具有清热解毒、消肿止痛的功效。长期以来，民间一直将其用于治疗。随着重楼应用的增加，野生资源日益短缺。因此，本研究比较了其他重楼属植物的主要活性，以寻找替代植物。
从中国三个省采集了 6 个种（云南重楼、大理重楼、红花重楼、巴山重楼、小花重楼和西藏重楼），并通过不同的测定方法比较了止血、细胞毒性和溶血活性。
在止血活性方面，除红花重楼外，所有植物均能显著缩短尾出血时间和凝血时间（P<0.05）。进一步的机制研究表明，它们可降低凝血酶原时间（PT）和部分凝血活酶时间（APTT），但对凝血时间（TT）无明显影响。红花重楼与云南重楼的细胞毒性相似（IC（50）：分别为 18.21 和 15.73μg/mL）。HD（50）用作溶血活性的指标。大理重楼和巴山重楼是最后显示出这种活性的植物，其值分别为 3.027 和 1.222mg/mL。
不同种的重楼具有不同的活性。大理重楼和巴山重楼可作为止血药物资源，红花重楼可作为抗肿瘤药物。
