Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China.
Pharm Biol. 2013 Oct;51(10):1321-5. doi: 10.3109/13880209.2013.790065. Epub 2013 Jul 16.
CONTEXT. Paris bashanensis Wang et Tang (Liliaceae) is widely used in traditional Chinese medicine for the treatment of injuries, fractures and hemorrhage in Hubei and Sichuan Province.
The n-BuOH extract of Paris bashanensis was investigated for hemostatic activity and chemical constituents in order to provide a basis for the application in folk use.
The n-BuOH extract of P. bashanensis was divided into three eluents (30, 50 and 70% EtOH) by macroporous adsorptive resin D101. The bleeding time of breaking tail hemostasis and clotting time of capillary and slide method in mice were used extensively to screen the hemostasis properties after repetitive administration of these three fractions (100 mg/kg, 50 mg/kg) for 5 days (total of 5 times, once per day). The chemical compounds were analyzed by HPLC-UV.
The inhibition rates in the bleeding time of 70, 50 and 30% n-BuOH ext. were 45, 32 and 21%, respectively. Using the slide method the decreasing rate of the clotting time of 70, 50 and 30% n-BuOH ext. were 71, 65 and 32% and in the experiment of capillary method, the inhibition rates were 43, 31 and 24%, respectively. A total of 70% n-BuOH ext. showed a high content of the pennogenin-type saponins by HPLC-UV.
The 70% n-BuOH ext. of P. bashanensis was found to contain high levels of pennogenin saponins, which may lead to a higher hemostatic activity. Combined with the hemostatic test, P. bashanensis could be used as a resource of hemostatic drug.
背景。巴山贝母（百合科）在湖北和四川广泛应用于传统中医治疗创伤、骨折和出血。
为了为民间应用提供依据，研究巴山贝母的正丁醇提取物的止血活性和化学成分。
用大孔吸附树脂 D101 将巴山贝母的正丁醇提取物分为三个洗脱液（30%、50%和 70%乙醇）。广泛采用小鼠尾断出血时间和毛细血管、玻片法凝血时间来筛选这三种馏分（100mg/kg、50mg/kg）连续给药 5 天（共 5 次，每天 1 次）后的止血作用。用 HPLC-UV 分析化合物。
70%、50%和 30%正丁醇提取物的出血时间抑制率分别为 45%、32%和 21%。用玻片法凝血时间减少率为 70%、50%和 30%正丁醇提取物分别为 71%、65%和 32%，在毛细管法实验中，抑制率分别为 43%、31%和 24%。70%正丁醇提取物通过 HPLC-UV 显示出较高含量的羽扇豆型皂苷。
发现巴山贝母的 70%正丁醇提取物含有高水平的羽扇豆型皂苷，这可能导致更高的止血活性。结合止血试验，巴山贝母可作为止血药物的资源。
