扩展革兰氏阳性菌的质粒分类系统，并确定人源、动物源和食品源金黄色葡萄球菌菌株中质粒的多样性。
Expansion of a plasmid classification system for Gram-positive bacteria and determination of the diversity of plasmids in Staphylococcus aureus strains of human, animal, and food origins.
机构信息
Area Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
出版信息
Appl Environ Microbiol. 2012 Aug;78(16):5948-55. doi: 10.1128/AEM.00870-12. Epub 2012 Jun 8.
Abstract
An expansion of a previously described plasmid classification was performed and used to reveal the plasmid content of a collection of 92 Staphylococcus aureus strains of different origins. rep genes of other genera were detected in Staphylococcus. S1 pulsed-field gel electrophoresis (PFGE) hybridizations were performed with 18 representative S. aureus strains, and a high number of plasmids of different sizes and organizations were detected.
摘要
对先前描述的质粒分类进行了扩展，并将其用于揭示来自不同来源的 92 株金黄色葡萄球菌菌株的质粒内容。在金黄色葡萄球菌中检测到了其他属的 rep 基因。对 18 株代表性金黄色葡萄球菌菌株进行了 S1 脉冲场凝胶电泳 (PFGE) 杂交，检测到了大量不同大小和结构的质粒。
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