Health Services Command, Turkish Armed Forces, Ankara, Turkey.
Ren Fail. 2012;34(7):921-5. doi: 10.3109/0886022X.2012.692752. Epub 2012 Jun 11.
This study was designed to investigate the possible beneficial effects of medical ozone therapy (OT), known as an immunomodulator and antioxidant, on the renal function, morphology, and biochemical parameters of oxidative stress in kidneys subjected to ischemia/reperfusion injury (IRI).
Thirty male Sprague-Dawley rats were classified into three groups: control, renal IRI, and renal IRI + OT. The IRI group was induced by bilateral renal ischemia for 60 min, followed by reperfusion for 6 h. After reperfusion, the kidneys and blood of rats were obtained for histopathologic and biochemical evaluation.
Renal IRI increased the tissue oxidative stress parameters (lipid peroxidation, protein oxidation, and nitrite plus nitrate) and decreased the antioxidant enzyme activities (superoxide dismutase and glutathione peroxidase). The serum neopterin levels showed correlation with oxidative stress parameters. All these parameters were brought to control values in the treatment group. Histopathologically, the kidney injury in the treatment group was significantly lesser than in the renal IRI group.
Our results clearly showed that OT has beneficial effect to protect kidney against IRI. The serum neopterin levels might be used as a marker to detect the degree of renal IRI.
本研究旨在探讨医用臭氧治疗（OT）作为一种免疫调节剂和抗氧化剂，对缺血/再灌注损伤（IRI）肾脏的肾功能、形态和氧化应激生化参数的可能有益影响。
30 只雄性 Sprague-Dawley 大鼠分为三组：对照组、肾 IRI 组和肾 IRI+OT 组。IRI 组通过双侧肾缺血 60 分钟诱导，然后再灌注 6 小时。再灌注后，获取大鼠的肾脏和血液进行组织病理学和生化评估。
肾 IRI 增加了组织氧化应激参数（脂质过氧化、蛋白质氧化和亚硝酸盐加硝酸盐），降低了抗氧化酶活性（超氧化物歧化酶和谷胱甘肽过氧化物酶）。血清中新蝶呤水平与氧化应激参数相关。在治疗组中，所有这些参数均恢复至对照组水平。组织病理学上，治疗组的肾脏损伤明显小于肾 IRI 组。
我们的结果清楚地表明，OT 对保护肾脏免受 IRI 具有有益作用。血清中新蝶呤水平可作为检测肾 IRI 程度的标志物。
